Transcriptomics

Dataset Information

0

The aryl hydrocarbon receptor in keratinocytes is essential for murine skin barrier integrity


ABSTRACT: The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor involved in adaptive cell functions, and highly active in the epidermis. AhR-ligands can accelerate keratinocyte differentiation, but a precise role for AhR in the skin barrier is unknown. We here show that transepidermal water loss (TEWL), a parameter of skin barrier integrity, is high in AhR-deficient (AhR-KO) mice. Experiments with conditionally AhR-deficient mouse lines identified keratinocytes as the major responsible cell population for high TEWL. Electron microscopy showed weaker inter-cellular connectivity in the epidermis of keratinocytes in AhR-KO mice, and gene expression analysis identified many barrier-associated genes as AhR targets. Moreover, AhR-deficient mice had higher inter-individual differences in their microbiome. Interestingly, removing AhR-ligands from the diet of wild-type mice mimicked AhR-deficiency regarding the impaired barrier. Vice versa, re-addition of the plant-derived ligand indole-3-carbinol (I3C) rescued the barrier deficiency even in aged mice. Our results suggest that functional AhR expression is critical for skin barrier integrity and that AhR represents a molecular target for the development of novel therapeutic approaches for skin barrier diseases, including dietary intervention.

ORGANISM(S): Mus musculus

PROVIDER: GSE80273 | GEO | 2016/08/31

SECONDARY ACCESSION(S): PRJNA318465

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2024-10-23 | GSE275323 | GEO
2012-12-01 | GSE42653 | GEO
2014-06-11 | E-GEOD-47607 | biostudies-arrayexpress
2022-02-28 | GSE189751 | GEO
2014-06-11 | E-GEOD-47944 | biostudies-arrayexpress
2016-11-13 | GSE72455 | GEO
2016-11-13 | GSE72449 | GEO
2022-10-04 | PXD025431 | Pride
2008-06-12 | E-GEOD-2955 | biostudies-arrayexpress
2014-06-11 | GSE47944 | GEO