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PRC2 preserves cellular plasticity and restricts intestinal secretory lineage commitment (ChIP-seq)

ABSTRACT: These data include the genome wide occupancy of histone modifications and target protein by ChIP sequencing in mouse crypt cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE80502 | GEO | 2017/01/16

SECONDARY ACCESSION(S): PRJNA319205

REPOSITORIES: GEO

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PRC2 preserves intestinal progenitors and restricts secretory lineage commitment.

Project description:Chromatin modifications shape cell heterogeneity by activating and repressing defined sets of genes involved in cell proliferation, differentiation and development. Polycomb-repressive complexes (PRCs) act synergistically during development and differentiation by maintaining transcriptional repression of common genes. PRC2 exerts this activity by catalysing H3K27 trimethylation. Here, we show that in the intestinal epithelium PRC2 is required to sustain progenitor cell proliferation and the correct balance between secretory and absorptive lineage differentiation programs. Using genetic models, we show that PRC2 activity is largely dispensable for intestinal stem cell maintenance but is strictly required for radiation-induced regeneration by preventing Cdkn2a transcription. Combining these models with genomewide molecular analysis, we further demonstrate that preferential accumulation of secretory cells does not result from impaired proliferation of progenitor cells induced by Cdkn2a activation but rather from direct regulation of transcription factors responsible for secretory lineage commitment. Overall, our data uncover a dual role of PRC2 in intestinal homeostasis highlighting the importance of this repressive layer in controlling cell plasticity and lineage choices in adult tissues.

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PRC2 preserves cellular plasticity and restricts intestinal secretory lineage commitment

Project description:This SuperSeries is composed of the SubSeries listed below.

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PRC2 preserves cellular plasticity and restricts intestinal secretory lineage commitment

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IL-17RA-signaling in Lgr5<sup>+</sup> intestinal stem cells induces expression of transcription factor ATOH1 to promote secretory cell lineage commitment.

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The Hox Transcription Factor Ubx stabilizes Lineage Commitment by Suppressing Cellular Plasticity

Project description:This SuperSeries is composed of the SubSeries listed below.

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Daxx maintains endogenous retroviral silencing and restricts cellular plasticity in vivo.

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