Genomics

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ChIP-exo data of the Streptomyces venezualae protein Sven6563 (RsrR)


ABSTRACT: We report the results of ChIP-Seq experiment investigating the Rrf2 family transciptional regulator RsrR (putativeily name Redox sensitive response regulator). The experiment utilised an in trans copy of rsrR with an N-terminal 3xFlag tag encoded on the plasmid pMS82 (containing a phiBT1 integration site). The experiment utilised beads associated with anti-flag antibodies, specific for the Flag tagged protein and a WT host strain. Exo nuclease treatment was carried prior to immunoprecipitation to improve the resolution of the binding site. We report >600 target binding sites for the RsrR regulon encompasing a broad range of functional targets with specific refernce to those associate with NADPH and NADH metabolism and synthesis.

ORGANISM(S): Streptomyces venezuelae

PROVIDER: GSE80818 | GEO | 2017/02/12

SECONDARY ACCESSION(S): PRJNA320079

REPOSITORIES: GEO

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