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Genomic analysis of human parthenogenetic haploid ESCs (hPGES), normal human ESCs(H9) and human forskin fibroblast

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE81025 | GEO | 2016/07/13

SECONDARY ACCESSION(S): PRJNA320304

REPOSITORIES: GEO

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mRNA expression data from human parthenogenetic haploid ESCs (hPGES), normal ESCs (H9) and human fibroblast

Project description:Here we report the derivation of human haploid ESCs from parthenogenetic haploid embryos. We used RNA-seq to compare the gene expression levels among human parthenogenetic haploid ESCs (hPGES), normal human ESCs (H9) and human forskin fibroblasts and identified that these cells express conventional ESCs pluripotent markers and most maternally imprinted genes were down-regulated.

2016-07-13 | GSE80988 | GEO

DNA methylation data from human parthenogenetic haploid ESCs (hPGES), normal human ESCs(H9) and human forskin fibroblast

Project description:Here we report the derivation of human parthenogenetic haploid ESCs which contain only one set of chromosome. These two cell lines, which we designated hPGES1 and hPGES2, show conventional ESCs and parthenogenetic-derived DNA methylation state.

2016-07-13 | GSE80226 | GEO

Genomic analysis of human parthenogenetic haploid ESCs (hPGES), normal human ESCs(H9) and human forskin fibroblast

Project description:Genomic analysis of human parthenogenetic haploid ESCs (hPGES), normal human ESCs(H9) and human forskin fibroblast

| PRJNA320304 | ENA

CNV anlysis of human parthenogenetic haploid ESCs (hPGES)

Project description:The genomic DNA sample of hPGES were compared to UCSC Human genome19 by CNV data. The data confirmed that the human parthenogenetic haploid ESCs sustained normal genome integrity

2016-07-13 | GSE80208 | GEO

Derivation of monkey parthenogenetic haploid embryonic stem cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-06-26 | E-GEOD-43980 | biostudies-arrayexpress

Derivation of monkey parthenogenetic haploid embryonic stem cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2013-06-26 | GSE43980 | GEO

G2/M transition for stabilizing mouse haploid ESCs

Project description:This SuperSeries is composed of the SubSeries listed below.

2014-11-07 | GSE55446 | GEO

Germline potential of parthenogenetic haploid mouse embryonic stem cells.

Project description:Haploid embryonic stem cells (ESCs) have recently been derived from parthenogenetic mouse embryos and offer new possibilities for genetic screens. The ability of haploid ESCs to give rise to a wide range of differentiated cell types in the embryo and in vitro has been demonstrated. However, it has remained unclear whether haploid ESCs can contribute to the germline. Here, we show that parthenogenetic haploid ESCs at high passage have robust germline competence enabling the production of transgenic mouse strains from genetically modified haploid ESCs. We also show that differentiation of haploid ESCs in the embryo correlates with the gain of a diploid karyotype and that diploidisation is the result of endoreduplication and not cell fusion. By contrast, we find that a haploid karyotype is maintained when differentiation to an extra-embryonic fate is forced by induction of Gata6.

| S-EPMC3424041 | biostudies-literature

Gene Expression Analysis of Cancer-Associated Fibroblast (CAF) compared to Normal Fibroblast (NF)

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-06-18 | E-GEOD-55871 | biostudies-arrayexpress

Self-diploidization of human haploid parthenogenetic embryos through the Rho pathway regulates endomitosis and failed cytokinesis.

Project description:A diploid genome is necessary for normal mammalian development, thus haploid parthenogenetic embryos undergo frequent self-diploidization during preimplantation development; however, the underlying mechanism is unclear. In this study, time-lapse recording revealed that human haploid parthenotes (HPs) undergo self-diploidization via failed cytokinesis (FC) and endomitosis (EM). The frequencies of FC/EM were significantly higher in HPs than in normal fertilized embryos (26.3% vs. 1.6%, P?<?0.01; 19.7% vs. 0, P?<?0.01), and above 90% of FC/EM occurred at the first cell cycle in HPs. Fluorescent in situ hybridization of chromosome 16,18 and X in HPs identified diploid recovery after the appearance of FC/EM, and FC/EM HPs showed improved blastocyst formation compared with non-FC/EM HPs (18.8% and 40.0% vs. 15.4%, P?>?0.05). In 66.7% of the 1-cell stage HPs, furrow ingression was not observed during the time for normal cleavage, and both immunostaining and gene expression analysis of 1-cell stage HPs revealed the absence or down-regulation of several key genes of the Rho pathway, which regulates cytomitosis. Our results suggested that the major mechanism for self-diploidization is Rho pathway inhibition leading to FC/EM in the first cell cycle, and fine-tuning of this signalling pathway may help to generate stable haploid embryos for stem cell biology studies.

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