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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type, ebr1 and EIP1-OX Transcriptomes

ABSTRACT: Crop breeding for board-spectrum disease resistance is the most profitable strategy to control diseases. However, knowledge on genes and mechanism of board-spectrum resistance is very limited. We fund a rice mutant ebr1 (enhanced blight and blast resistance 1) showed broad-spectrum resistance to both bacterial Xanthomonas oryzae pv. oryzae and fungal Magnaporthe oryzae associated with spontaneous programmed cell death (PCD), autoimmunity activation and stunting. EBR1 is located at the chromosome centromere and encodes a previously unknown RING-type protein with E3 ubiquitin ligase activity. Extensive yeast two-hybrid screening identified one EIP (EBR1-interacting) protein, EIP1, which belongs to the BAG (Bcl-2 associated athanogene) family that have been associated with stress responses and modulation of cell death.cross kingdoms. We showed that EIP1 is a specially target of EBR1 and is a key player activating PCD and immunity including the salicylic acid and jasmonate defense pathways in rice. Its function is suppressed EBR1-mediated degradation through ubiquitination. EIP1-RNAi could restore the PCD and disease resistance phenotypes of ebr1, while EIP1 overexpression caused strong PCD and enhanced resistance to rice pathogens likely ebr1. Together, our study reveals a unique E3 ligase-BAD protein module that orchestrates autoimmunity and trade-off between defense and growth in rice.

ORGANISM(S): Oryza sativa Japonica Group

PROVIDER: GSE81108 | GEO | 2016/11/17

SECONDARY ACCESSION(S): PRJNA320536

REPOSITORIES: GEO

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Project description:Bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), is the most destructive bacterial disease of rice. The cloned rice gene Xa21 confers resistance to a broad spectrum of Xoo races. To identify other genes involved in Xa21-mediated immunity, a whole-genome oligonucleotide microarray of rice was used to profile the expression of rice genes between incompatible interactions and mock treatments at 0, 4, 8, 24, 72 and 120 hours post inoculation (hpi) or between incompatible and compatible interactions at 4 hpi, respectively. A total of 441 differentially expressed genes, designated as XDGs (Xa21 mediated Differentially expressed Genes), were identified. Based on their functional annotations, the XDGs were assigned to 14 categories some of which encode resistance/defense related proteins and signaling regulators. Interestingly, most signaling-related genes were down-regulated at 4 and 8 hpi, suggesting that negative regulation of cellular signaling may play a role in the Xa21-mediated defense response. Moreover, a number of pathogenesis-related genes were induced at 72 and 120 hpi. Comparison of expression profiles mediated by other resistance genes revealed an interesting common responses in the plant immune system. Comparison analyses also suggest possible overlaps with hormone signaling pathways and with defense systems including basal defense and hypersensitive responses. We investigated the transcriptional characteristics of Xa21-mediated defense responses in rice using whole-genome oligonucleotide microarray, by comparing the expression profiles between incompatible interactions and mock treatments at five time-points and between incompatible and compatible interactions at 4 hours post inoculation. Microarray hybridization at each time point was carried out in three replications including two biological replications and one technological replication. To detect the genes that are significantly regulated and to eliminate those that have inconsistent expression data among replicated experiments, a cutoff of 1.5 fold change and 1.96 for intensity-dependent Z-scores were required in at least two experimental samples.

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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type, ebr1 and EIP1-OX Transcriptomes

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