Transcriptomics

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Identification and characterization of a glycosulfatase-encoding gene cluster in Bifidobacterium breve UCC2003


ABSTRACT: A bacterial nursling stool isolate, Bifidobacterium breve UCC2003, encodes two putative sulfatases. The sulfated monosaccharide N-acetylglucosamine-6-sulfate (GlcNAc-6-S) was shown to support growth of B. breve UCC2003, while three other tested sulfated monosaccharides, N-acetylglucosamine-3-sulfate, N-acetylgalactosamine-3-sulfate and N-acetylgalactosamine-6-sulfate, did not. Using a combination of transcriptomic and functional genomic approaches, a gene cluster, designated ats2, was shown to be specifically required for GlcNAc-6-S metabolism. Transcription of the ats2 cluster is shown to be regulated by a ROK-family transcriptional repressor. Expression profiling by array

ORGANISM(S): Bifidobacterium breve UCC2003

PROVIDER: GSE81240 | GEO | 2016/05/10

SECONDARY ACCESSION(S): PRJNA321030

REPOSITORIES: GEO

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