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The effect of EPO on gene expression signatures of hepatocellular carcinoma cells

ABSTRACT: To gain further insight into the downstream effects of EPO signaling on HCC cells, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to illustrate EPO-induced the proliferation of HCC cells. 1×10e6 7721-EPOR cells were plated in 6 cm dishes and starved for 8 hours and then treated with either NS or EPO (10 IU/ml) for 16 hours. Total RNA was extracted and profiled using oligo microarrays. Differentially expressed genes were then identified through fold change as well as P value calculated with t-test. The threshold set for up- and down-regulated genes was a fold change>= 2.0 and a P value<= 0.05. Afterwards, GO analysis and KEGG analysis were applied to determine the roles of these differentially expressed mRNAs. EPO treated HCC cells up-regulated 14 genes expression and down-regulated 29 gene expression compared to control cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE81310 | GEO | 2017/05/01

SECONDARY ACCESSION(S): PRJNA321256

REPOSITORIES: GEO

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