Transcriptomics

Dataset Information

0

Systems genetics reveals a transcriptional network associated with susceptibility in the maize-gray leaf spot pathosystem


ABSTRACT: Background: Maize plants developed typical gray leaf spot disease (GLS) symptoms initiating at the lower leaves and progressing to upper leaves through the season. Leaf material was collected at 77 days after planting, at which stage there were a large number of GLS disease necrotic lesions on lower leaves (8% surface area on average determined by digital image analysis), but very few lesions and only at chlorotic stage on leaves above the ear (average of 0.2% lesion surface area). Method:To collect material that reflected a difference between C.zeina infected B73 leaves and control B73 leaf material, samples were collected from two lower GLS infected leaves (second and third leaf internode below ear) , and two upper leaves with minimal GLS symptoms (second and third internode above ear), respectively. The two lower leaves from each plant were pooled prior to RNA extraction, and the two upper leaves from each plant were pooled prior to RNA extraction. Upper and lower leaf samples from three maize B73 plants were subjected to RNA sequencing individually. The three maize plants were selected randomly as one plant per row from three rows of ten B73 plants each. Result: A systems genetics strategy revealed regions on the maize genome underlying co-expression of genes in susceptible and resistance responses, including a set of 100 genes common to the susceptible response of sub-tropical and temperate maize.

ORGANISM(S): Zea mays

PROVIDER: GSE81344 | GEO | 2016/10/15

SECONDARY ACCESSION(S): PRJNA321325

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2020-02-14 | GSE137198 | GEO
2017-06-14 | GSE94442 | GEO
2020-06-29 | GSE152107 | GEO
2009-12-11 | GSE18011 | GEO
2014-03-04 | GSE55524 | GEO
2012-11-01 | E-GEOD-40678 | biostudies-arrayexpress
2010-12-10 | GSE25994 | GEO
2012-11-01 | GSE40678 | GEO
2010-05-18 | E-GEOD-18011 | biostudies-arrayexpress
2016-02-09 | E-GEOD-77657 | biostudies-arrayexpress