Transcriptomics

Dataset Information

0

Near-tetraploid cells exhibit chromosome instability triggered by replication stress and enhanced invasive capabilities [expression]


ABSTRACT: A considerable proportion of tumors exhibit aneuploid karyotypes, likely resulting from the loss of chromosomes following whole genome duplication. Here, by using isogenic diploid and near-tetraploid clones derived from the same parental cell line, we aimed at exploring how polyploidization affects cellular functions and how tetraploidy generates chromosome instability. Gene expression profiling in near-tetraploid clones revealed a significant enrichment of genes involved in replication stress. This increased level of replication stress resulted in DNA damage, greater sensitivity to S-phase checkpoint inhibitors, and impaired proliferation caused by a cell cycle delay during S-phase. Additionally, replication stress promoted higher levels of intercellular heterogeneity and ongoing genomic instability, which we observed in the form of abnormal anaphases and prometaphase events. Finally, our data unveiled that near-tetraploid cells displayed increased migratory and invasive capacities, both in vitro and in primary colorectal tumors, thus providing physiological advantages to the cancer cell.

ORGANISM(S): Homo sapiens

PROVIDER: GSE81394 | GEO | 2017/05/10

SECONDARY ACCESSION(S): PRJNA321436

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2017-05-10 | GSE81303 | GEO
| PRJNA321435 | ENA
2006-03-01 | E-TABM-70 | biostudies-arrayexpress
2014-09-07 | E-GEOD-42306 | biostudies-arrayexpress
2014-09-07 | GSE42266 | GEO
2014-09-07 | GSE42306 | GEO
2014-09-07 | E-GEOD-42266 | biostudies-arrayexpress
| PRJNA321436 | ENA
| PRJNA321437 | ENA
2016-09-01 | GSE86101 | GEO