Transcriptomics

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RNA-seq analysis of hsf-1 mutant in C. elegans larval development


ABSTRACT: To understand the function and regulation of the C. elegans heat shock factor (HSF-1) in larval development, we have used ChIP-seq to analyze the occupancy of HSF1 and RNA Pol II in L2 larvae and young adult (YA) animals grown at 20°C or upon heat shock at 34°C for 30 min. In addition, we have used RNA-seq to analyze the transcriptomes of wild type (N2), hsf-1(ok600) mutants and hsf-1(ok600); rmSi1[hsf-1::gfp] L2 larvae grown at 20°C and characterized the gene expression change by heat shock in wild type (N2) animals at L2 stage.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE81522 | GEO | 2016/10/12

SECONDARY ACCESSION(S): PRJNA321851

REPOSITORIES: GEO

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