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RNAs enriched at protrusions of control or APC knockdown fibroblasts

ABSTRACT: We used a fractionation scheme to isolate protrusions and cell bodies from control or APC knockdown fibroblasts, which were induced to extend protrusions in response to addition of lysophosphatidic acid (LPA). Four replicate protrusion and cell body samples for each cell type were analyzed by RNA-Seq. To identify RNAs enriched in protrusions, a protrusion/cell body ratio was calculated. We find that ca. 7% of detected RNAs were enriched in protrusions of control cells. Knockdown of APC reduced the protrusion enrichement of a subset of these RNAs, indicating that they are dependent on APC for their localization. Localization of the remaining RNAs was not significantly affected upon APC knockdown suggesting that their localization is mediated by an APC-independent mechanism.

ORGANISM(S): Mus musculus

PROVIDER: GSE82229 | GEO | 2017/10/11

SECONDARY ACCESSION(S): PRJNA324403

REPOSITORIES: GEO

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Mus musculus

Project description:RNAs enriched at protrusions of control or APC knockdown fibroblasts

| PRJNA324403 | ENA

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