Transcriptomics

Dataset Information

0

An improved method for identifying biological circular RNAs


ABSTRACT: Purpose: We are using the illumina sequencing to compare the false positive and true positive circular RNA findings to confine the method to detect the true circular RNAs Methods: The testis whole transcriptome profiling was generated from 4-week mouse testis using illumina Nextseq, duplicated. The sequence reads that passed quality filters were analyzed at the transcript isoform level with TopHat followed by Cufflinks. Results: our data suggest that circular RNAs identified based on junction sequences in the RNA-seq reads, especially those from Illumina Hiseq sequencing, mostly result from template-switching events during reverse transcription by MMLV-derived reverse transcriptases. It is critical to employ reverse transcriptases lacking terminal transferase activity (e.g., MonsterScript) to construct sequencing library or perform RT-PCR for identification and quantification of true circular RNAs. Conclusions: Our study represents the first detailed analysis of retinal transcriptomes, with biologic replicates, generated by RNA-seq technology. The optimized data analysis workflows reported here should provide a framework for comparative investigations of expression profiles. Our results show that NGS offers a comprehensive and more accurate quantitative and qualitative evaluation of mRNA content within a cell or tissue. We conclude that RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biologic functions.

ORGANISM(S): Mus musculus synthetic construct

PROVIDER: GSE83992 | GEO | 2016/07/05

SECONDARY ACCESSION(S): PRJNA327756

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2016-07-05 | E-GEOD-83992 | biostudies-arrayexpress
2020-04-22 | GSE149061 | GEO
2015-09-25 | GSE72127 | GEO
2022-08-10 | GSE209924 | GEO
| PRJNA349265 | ENA
| PRJNA420752 | ENA
| PRJNA861237 | ENA
2023-08-24 | GSE230506 | GEO
2019-09-24 | GSE123365 | GEO
2021-10-25 | GSE186464 | GEO