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Compared performance of Affymetrix HTA arrays and Illumina RNAseq for the analysis of tumours [Affymetrix]

ABSTRACT: Here we compared the performance of Affymetrix HTA 2.0 microarray and Illumina 2000 RNA-sequencing techniques on the clinical samples collected from patients with lung squamous cell carcinoma.

ORGANISM(S): Homo sapiens

PROVIDER: GSE84784 | GEO | 2017/06/09

SECONDARY ACCESSION(S): PRJNA331143

REPOSITORIES: GEO

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Compared performance of Affymetrix HTA arrays and Illumina RNAseq for the analysis of tumours [RNA-seq]

Project description:Here we compared the performance of Affymetrix HTA 2.0 microarray and Illumina 2000 RNA-sequencing techniques on the clinical samples collected from patients with lung squamous cell carcinoma.

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Compared performance of Affymetrix HTA arrays and Illumina RNAseq for the analysis of tumours

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Compared performance of Affymetrix HTA arrays and Illumina RNAseq for the analysis of tumours

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Comparison of Affymetrix and NuGEN amplification methods for HTA 2.0 microarray

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Performance comparison of Affymetrix and Illumina microarray technologies

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Transcriptome data of multiple sclerosis patients receiving fingolimod therapy [HTA-2_0, CD4+ cells, gene level]

Project description:We analyzed the gene expression patterns of different blood cell types before and during fingolimod treatment in a group of patients with relapsing-remitting multiple sclerosis (RRMS). Affymetrix Human Transcriptome Arrays (HTA) 2.0 were used to obtain gene expression profiles of immune cell populations from 10 RRMS patients within the first 3 months of fingolimod treatment. This GEO entry provides the Affymetrix HTA 2.0 microarray data of peripheral blood CD4+ cells, preprocessed according to the gene level workflow. EDTA blood samples were taken from all patients immediately before first and second fingolimod administration as well as after 3 months of therapy. Total RNA of CD4+ cells positively selected from each blood sample was extracted, labeled and hybridized to high-density Affymetrix HTA 2.0 microarrays to quantify the transcript levels.

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Transcriptome data of multiple sclerosis patients receiving fingolimod therapy [HTA-2_0, CD8+ cells, gene level]

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