Genomics

Dataset Information

0

Small RNA and PARE sequencing in rice (photoperiod sensitive male sterility lines and wild type) panicles under long-day and short-day conditions


ABSTRACT: Purpose: The goals of this study are to detect the small RNAs generated from the PMS1T gene and the cleavage sites mediated by small RNAs (microRNAs or phasiRNAs). Methods: The rice panicles were collected in liquid nitrogen and their total RNA were extracted for library construction and subsequent sequencing on an Illumina HiSeq 2000. Results: Using an optimized data analysis workflow, we mapped a series of small RNAs generated from the PMS1T region of the rice genome; most of these small RNAs were 21-nt phasiRNAs. The results from PARE sequencing validated the cleavage site on the PMS1T transcript, with the cleavage mediated by miR2118. Conclusions: Our results show that the 21-nt phasiRNA generated from the PMS1T transcript are associated with photoperiod-sensitive male sterility in rice.

ORGANISM(S): Oryza sativa Japonica Group

PROVIDER: GSE84885 | GEO | 2016/12/18

SECONDARY ACCESSION(S): PRJNA335548

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2022-08-30 | GSE191268 | GEO
2020-09-24 | GSE149800 | GEO
2012-03-29 | E-GEOD-22763 | biostudies-arrayexpress
2024-01-01 | GSE232779 | GEO
2012-03-30 | GSE22763 | GEO
2018-12-17 | GSE122449 | GEO
2012-07-03 | GSE26405 | GEO
2016-04-18 | GSE48346 | GEO
2012-07-02 | E-GEOD-26405 | biostudies-arrayexpress
2024-05-15 | GSE254581 | GEO