F. verticillioides cultures on dissected maize tissues
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ABSTRACT:
ORGANISM(S): Fusarium verticillioides
PROVIDER: GSE8535 | GEO | 2007/12/31
SECONDARY ACCESSION(S): PRJNA101671
REPOSITORIES: GEO
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Project description:F. verticillioides cultures on dissected maize tissues
| PRJNA101671 | ENA
Project description:Fusarium verticillioides is a detrimental fungus that can contaminate maize grains with mycotoxins that are harmful to human and animal health. Breeding and growing resistant genotypes is one alternative to reduce contamination and subsequent production of mycotoxins by this fungus. However, little is known about the resistant mechanism relevant to breeding in this pathosystem. Therefore, our aim was to identify genes and metabolites that may be related to Fusarium ear rot resistance using resistant and susceptible maize inbreds. Kernels of the resistant inbred showed significantly reduced disease severity, and reduced levels of total fumonisin and ergosterol content compared with the susceptible one. Gene expression data were obtained from microarray hybridizations using F. verticillioides inoculated and non inoculated maize kernels. Differentially expressed sequences were identified and classified into 36 functional categories. Most of the differentially expressed genes were assigned to the categories “protein, RNA, DNA, stress, transport, signaling and cell metabolism”. These genes encode for PR proteins, detoxification and primary metabolism enzymes. Fungal inoculation did not produce considerable changes in gene expression and metabolites in the resistant L4637 inbred, probably due to a preformed or constitutive resistance mechanism. Defense-related genes were induced or repressed in kernels of the susceptible inbred L4674, responding specifically to the pathogen infection. The qRT-PCR in infected silks showed that glucanase, lipid transfer, xylanase inhibitor, PR1 and 26S proteosome transcripts had higher expression ratios in the susceptible line compared to the resistant one in response to fungal infection. Through this study, a global view of differential genes expressed and metabolites concentration during resistance and susceptibility to F. verticillioides inoculation has been obtained, giving additional information about the mechanisms and pathways conferring resistance to this important disease in maize. Global view of differential genes expressed during resistance and susceptibility to F. verticillioides inoculation. Two maize inbred lines : one resistant (L4637) and one susceptible (L4674) to F. verticillioides infection. Two-condition experiment, Inoculated (I) vs. non-inoculated (NI) lines. Biological replicates: 3 . One replicate per array.
2013-08-17 | E-GEOD-40288 | biostudies-arrayexpress
Project description:Fusarium verticillioides is a detrimental fungus that can contaminate maize grains with mycotoxins that are harmful to human and animal health. Breeding and growing resistant genotypes is one alternative to reduce contamination and subsequent production of mycotoxins by this fungus. However, little is known about the resistant mechanism relevant to breeding in this pathosystem. Therefore, our aim was to identify genes and metabolites that may be related to Fusarium ear rot resistance using resistant and susceptible maize inbreds. Kernels of the resistant inbred showed significantly reduced disease severity, and reduced levels of total fumonisin and ergosterol content compared with the susceptible one. Gene expression data were obtained from microarray hybridizations using F. verticillioides inoculated and non inoculated maize kernels. Differentially expressed sequences were identified and classified into 36 functional categories. Most of the differentially expressed genes were assigned to the categories “protein, RNA, DNA, stress, transport, signaling and cell metabolism”. These genes encode for PR proteins, detoxification and primary metabolism enzymes. Fungal inoculation did not produce considerable changes in gene expression and metabolites in the resistant L4637 inbred, probably due to a preformed or constitutive resistance mechanism. Defense-related genes were induced or repressed in kernels of the susceptible inbred L4674, responding specifically to the pathogen infection. The qRT-PCR in infected silks showed that glucanase, lipid transfer, xylanase inhibitor, PR1 and 26S proteosome transcripts had higher expression ratios in the susceptible line compared to the resistant one in response to fungal infection. Through this study, a global view of differential genes expressed and metabolites concentration during resistance and susceptibility to F. verticillioides inoculation has been obtained, giving additional information about the mechanisms and pathways conferring resistance to this important disease in maize.
2013-08-17 | GSE40288 | GEO
Project description:Fusarium verticillioides (F. verticillioides) stalk rot is one of the most devastating diseases of maize that causes significant yield losses and poses potential security concerns of foods worldwide. The underlying mechanisms of maize plants regulating defense against the disease remain poorly understood. Here, integrative proteomic and transcriptomic analyses were employed to identify pathogenies-related protein genes by comparing differentially expressed genes (DEGs) and proteins (DEPs) in maize stalks after inoculated with F. verticillioides.
2022-11-04 | PXD037964 |
Project description:An gene-expression comparison of the wild type and strain delta-fst1 of Fusarium verticillioides grown on autoclaved maize kernels for six days was conducted.
2015-02-19 | GSE66044 | GEO
Project description:An gene-expression comparison of the wild type and strain delta-fst1 of Fusarium verticillioides grown on autoclaved maize kernels for six days was conducted. Comparison of gene-expression between the wild type and strain delta-fst1 of Fusarium verticillioides grown on autoclaved maize kernels for six days. For each strain, RNA was isolated from four biological replicates. Sequences was obtain by HiSeq Illumina sequencer.
2015-02-19 | E-GEOD-66044 | biostudies-arrayexpress
Project description:Investigation of whole genome gene expression level changes over time of Fusarium verticillioides wild-type in liquid fumonisin inducing media (GYAM) Fusarium verticillioides produces a polyketide derived mycotoxin, fumonsin, over time in liquid media, Proctor et al, Co-expression of 15 contiguous genes delineates a fumonisin biosynthetic gene cluster in Gibberella moniliformis, Fungal Genetics and Biology, 38:237-249 Wild type F. verticillioides strain 7600 cultured for two and five days in liquid GYAM.
2012-05-20 | E-GEOD-34168 | biostudies-arrayexpress
Project description:Fusarium verticillioides is one of the most important pathogens of maize, causing rots and producing fumonisin mycotoxins during infection. Ingestion of fumonisin-contaminated corn causes underperformance and even fatal toxicity in livestock and is associated with neural tube birth defects, growth stunting in children and some cancers. StuA, an APSES class transcription factor, is commonly a major developmental transcriptional regulator in fungi. It has been shown to regulate crucial developmental processes, such as sporulation, virulence and mycotoxin synthesis among others. In this study, the role of FvSTUA in F. verticillioides was examined by characterizing ∆FvstuA deletion mutants functionally and transcriptomally. The deletion mutants exhibited slower vegetative growth, stunted aerial hyphae and significant reductions in microconidiation. Macroconidiation and hydrophobicity of the deletion strains were reduced as well. Additionally, fumonisin production by and virulence of the deletion mutants were greatly reduced. Transcriptomic analysis revealed downregulation of expression of several genes in the fumonisin and fusarin C biosynthetic clusters and differential expression of genes involved in conidiation and virulence. Nuclear localization of FvSTUA-tdTomato supported the likely function of FvSTUA as a transcription factor. Together, our results indicate that FvSTUA plays a global role in transcriptional regulation in F. verticillioides influencing morphogenesis, toxin production and virulence.
2020-11-01 | GSE144966 | GEO
Project description:Expression analysis of Fusarium verticillioides wild-type cultured in liquid media
| PRJNA149717 | ENA
Project description:Expression analysis of wild type Fusarium verticillioides cultured in liquid media
| PRJNA117679 | ENA