Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MiRNA expression data from cultivated Human Thymic Epithelial Cells (hTECs) in comparison with cultivated Human Epidermal derived Keratinocytes (hEKs)

ABSTRACT: The thymic epithelium and the epidermis display many common features despite their different structure, function and embryonic origin. We used microarrays to uncover differences and similarities in the miRNA expression profile of hTECs and hEKs and investigate their significance for the peculiar organization of the thymic structure.

ORGANISM(S): Homo sapiens

PROVIDER: GSE85480 | GEO | 2018/07/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression data from cultivated Human Thymic Epithelial Cells (hTECs) in comparison with cultivated Human Epidermal derived Keratinocytes (hEKs)

Project description:The thymic epithelium and the epidermis display many common features despite their different structure, function and embryonic origin. We used microarrays to uncover differences and similarities in the gene expression profile of hTECs and hEKs in vitro and investigate their significance for thymic structure and function.

2018-07-31 | GSE85479 | GEO

Gene regulation in human epidermal keratinocytes by a Rho kinase inhibitor (Y-27632)

Project description:Keratinocytes are a targeted cells for infection of human papillomaviruses (HPV). But, there are very limited information for gene regulation of human karatinocytes by HPV infection. Y-27632, a rho-kinase inhibitor, stimulates proparation of keratinocytes. We identified genes, which are regulated by a drug for karatinocyte proparation (Y-27632). mRNA profiles of primary HEKs regulated by a rho kinase inhibitor (Y-27632, Y-drug) were generated by microarray analysis using Illumina BeadChip.

2014-03-31 | E-GEOD-52515 | biostudies-arrayexpress

RNA-Seq of cultivated (i) bulk human thymic epithelial cells (hTECs) (ii) cortical hTECs (iii) medullary hTECs (iv) human thymic interstitial cells (hTICs)

Project description:Transcriptomic profiling of cultivated human thymic epithelial cells and thymic interstitial cells.

2020-10-28 | E-MTAB-9641 | biostudies-arrayexpress

Effects of hydroquinone on whole-genome scale transcription of human epidermal keratinocytes.

Project description:Hydroquinone (HQ) is a well-known phenolic compound that causes chemical leukoderma. Hydroquinone was once used as photographic developers for films and papers, and it is widely used as a topical application in skin whitening to reduce the color of skin. Recently, the United States Food and Drug Administration (US FDA) banned all over-the-counter products with hydroquinone due to its potential toxicity. Topical cream with hydroquinone up to 4% should be prescribed and used with specific caution. In this study, to elucidate the toxicological role of keratinocyte in the pathogenesis of chemical leukoderma, we analyzed genome-wide transcriptional data of cultured human epidermal keratinocytes (HEKs) treated with sub-cytotoxic hydroquinone concentration.

2019-07-21 | GSE126721 | GEO

Gene expression profile of primary epidermal keratinocytes (HEKs) stimulated with lawsone

Project description:The study aimed to interrogate whether the 1,4-naphtoquinone derivative lawsone activates the aryl hydrocarbon receptor (AhR) downstream transcriptional program in keratinocytes and is capable of modulating skin homeostasis. The study has been performed on human primary epidermal keratinocytes (HEKs), which represent the first line of defense against exogenous molecules. HEK cells were treated with the vehicle control DMSO (control), stimulated with lawsone or with the TLR2 ligand Pam2CSK4. Transcriptional profiles of HEK cells were studied to investigate the regulation of AhR -related genes, Nrf2-related genes and epidermal differentiation and keratin genes. We demonstrated that lawsone was sensed by keratinocytes and activated AhR. In particular, lawsone efficiently activated the transcriptional program of AhR and promoted keratinocyte differentiation.

2019-08-06 | GSE99901 | GEO

Expression data of human tympanic membrane cells and epidermal keratinocytes [miRNA]

Project description:The data set compares the microRNA expression of two types of keratinocytes from the tympanic membrane and epidermis of the body. Healthy human samples were harvested for the analysis. Cells were cultivated to evaluate the effect of keratinocytes only. Few Chip Ids (11) were considered differentially expressed between the cell types.

2016-05-31 | GSE76673 | GEO

Expression data comparing human foreskin keratinocytes to epithelial derivatives of human embryonic stem cells (H9)

Project description:Whole genome expression array comparison of primary keratinocytes to retinoic acid-differentiated hES cell derivatives Cells were differentiated according to Metallo et al. Stem Cells 2008 A 3 chip study was conducted using competitive hybridization comparing pFKs to hEKs obtained in 3 independent differentiation experiments

2010-05-06 | E-GEOD-17265 | biostudies-arrayexpress

Expression data of human tympanic membrane cells and epidermal keratinocytes [mRNA]

Project description:The data set compares the gene expression of two types of keratinocytes from the tympanic membrane and epidermis of the body. Healthy human samples were harvested for the analysis. Cells were cultivated to evaluate the effekt of keratinocytes only. 626 Chip Ids considered differentially expressed between the cell types. Enrichment tests show genes related to migration are over-represented in the highly expressed genes of TMK vs. EK.

2014-11-04 | GSE62939 | GEO

Expression data of human tympanic membrane cells and epidermal keratinocytes

2014-11-04 | E-GEOD-62939 | biostudies-arrayexpress

Epidermal differentiation: basal vs. suprabasal keratinocytes

Project description:In epidermal differentiation basal keratinocytes detach from the basement membrane, stop proliferating, and express a new set of structural proteins and enzymes, which results in an impermeable protein/lipid barrier that protects us. To define the transcriptional changes essential for this process, we purified large quantities of basal and suprabasal cells from human epidermis, using the expression of b4 integrin as the discriminating factor. The expected expression differences in cytoskeletal, cell cycle and adhesion genes confirmed the effective separation of the cell populations. Using DNA microarray chips, we comprehensively identify the differences in genes expressed in basal and differentiating layers of the epidermis, including the ECM components produced by the basal cells, the proteases in both the basal and suprabasal cells, and the lipid and steroid metabolism enzymes in suprabasal cells responsible for the permeability barrier. We identified the signaling pathways specific for the two populations, and found two previously unknown paracrine and one juxtacrine signaling pathway operating between the basal and suprabasal cells. Furthermore, using specific expression signatures, we identified a new set of late differentiation markers and mapped their chromosomal loci, as well as a new set of melanocyte-specific markers. The data represent a quantum jump in understanding the mechanisms of epidermal differentiation. Basal keratinocytes are defined as integrin b4-positive. Suprabasal sample contains all melanocytes as well. Basal (integrin b4+), suprabasal (b4-) and total epidermal keratinocytes. In duplicate, from 2 independent donors, breast reduction surgery.

2010-12-15 | E-GEOD-26059 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data