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An automatec microwell platform for large-scale single cell RNA-seq.

ABSTRACT: We report an automated microwell array platform for single cell RNA-seq with significantly improved performance over previous implementations. We demonstrate cell capture efficiencies of >50%, compatibility with commercially available barcoded mRNA capture beads, and parallel expression profiling from thousands of individual cells. We apply our system to comprehensively assess heterogeneity in gene expression of patient-derived glioma neurospheres and uncover subpopulations similar to those observed in human glioma tissue.

OTHER RELATED OMICS DATASETS IN: PRJNA338815

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE85575 | GEO | 2016/08/13

SECONDARY ACCESSION(S): PRJNA338815

REPOSITORIES: GEO

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