Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

LncRNA and mRNA Expression in aortic smooth muscle cells with Srf knockout or Myocd over-expression

ABSTRACT: LncRNA and mRNA expression profiles in aortic smooth muscle cells with Srf knockout or Myocd over-expression were analyzed by Arraystar Mouse LncRNA Microarray V3.0.

ORGANISM(S): Mus musculus

PROVIDER: GSE85930 | GEO | 2017/03/08

SECONDARY ACCESSION(S): PRJNA339784

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Arraystar human lncRNA microarray V3.0

Project description:Arraystar human lncRNA microarray V3.0

| PRJNA760918 | ENA

Transcription factors MYOCD, SRF, Mesp1 and SMARCD3 significantly enhance the cardio-inducing effect of GATA4, TBX5, and MEF2C during direct cellular reprogramming

Project description:Transient over-expression of defined combinations of master regulator genes can effectively induce cellular reprogramming: the acquisition of an alternative predicted phenotype from a differentiated cell lineage. This can be of particular importance in cardiac regenerative medicine wherein the heart lacks the capacity to heal itself, but simultaneously contains a large pool of fibroblasts. In this study we determined the cardio-inducing capacity of ten transcription factors to actuate cellular reprogramming of mouse embryonic fibroblasts into cardiomyocyte-like cells. Over-expression of transcription factors MYOCD and SRF alone or in conjunction with Mesp1 and SMARCD3 significantly enhanced the basal but necessary cardio-inducing effect of the previously reported GATA4, TBX5, and MEF2C. In particular, combinations of five or seven transcription factors significantly enhanced the activation of cardiac reporter vectors, and induced an upregulation of cardiac-specific genes. Global gene expression analysis also demonstrated a significantly greater cardio-inducing effect when the transcription factors MYOCD and SRF were used. Detection of cross-striated cells was highly dependent on the cell culture conditions and was enhanced by the addition of valproic acid and JAK inhibitor. Although we detected Ca2+ transient oscillations in the reprogrammed cells, we did not detect significant changes in resting membrane potential or spontaneously contracting cells. This study further elucidates the cardio-inducing effect of the transcriptional networks involved in cardiac cellular reprogramming, contributing to the ongoing rational design of a robust protocol required for cardiac regenerative therapies. Mouse embryonic fibroblasts were transduced lentiviruses allowing the inducible overexpression of three unique sets of transcription factors and negative control. A total of four experimental groups which included three biological replicates in each.

2013-03-31 | E-GEOD-44401 | biostudies-arrayexpress

Transcription factors MYOCD, SRF, Mesp1 and SMARCD3 significantly enhance the cardio-inducing effect of GATA4, TBX5, and MEF2C during direct cellular reprogramming

2013-03-31 | GSE44401 | GEO

Differentially expressed lncRNA&mRNA between macrophage M0 and macrophage M2 cells

Project description:To compare lncRNA&mRNA expression diffrence between macrophage M0 and macrophage M2 cells, we performed microarray using Arraystar Human LncRNA + mRNA Microarray v3.0 (8×60K) platform

2021-02-28 | GSE144789 | GEO

HNF1A-AS1, as competing endogenous RNA (ceRNA), is involved in VEGF-C-mediated PI3K signaling pathway in gastric cancer

Project description:To investigate the effect of lncRNA on theVEGF-C-mediated cancer development , genome-wide lncRNA were analyzed by Arraystar Human LncRNA microarray V3.0 upon knockdown VEGF-C in MKN-45gastric cancer cells.

2020-04-19 | GSE97967 | GEO

LncRNA expression profile in triple negative breast cancer (TNBC)

Project description:To identify novel TNBC-relevant lncRNAs, we performed lncRNA microarray analysis using 5 TNBC tissues and their matched adjacent non-cancerous tissues by Arraystar Human LncRNA Microarray V3.0.

2021-07-01 | GSE156144 | GEO

Caveolin-1 promotes the human trophoblasts cells invasion through FAK signaling pathway in the early placental development

Project description:mRNA expression profiles in the placenta tissues from different developmental stages in mice were analyzed by Arraystar Mouse LncRNA Microarray V3.0.

2018-03-22 | GSE112163 | GEO

Long non-coding RNAs in cutaneous squamous cell carcinoma

Project description:The expression of lncRNAs in cutaneous squamous cell carcinoma (cSCC) were determined with a commercially available microarray (Arraystar Inc.,Rockville, Maryland USA). The Arraystar human lncRNA Microarray V3.0 containing 30,586 LncRNAs and 26,109 coding transcripts (mRNAs)

2016-04-13 | GSE74859 | GEO

Identification of LncRNAs and mRNAs involved in adipogenesis

Project description:Genome wide lncRNA and mRNA expression profiling at day 0 and day 4 after induction of adipocyte differentiation in C3H10T1/2 and 3T3-L1 cells were analyzed by Arraystar Mouse LncRNA Microarray V3.0

2021-12-15 | GSE190733 | GEO

Long noncoding RNA expression profile of breast cancer tissues pre- and post-neoadjuvant chemotherapy

Project description:To identify the differential expression of lncRNAs, we performed the lncRNA microarray analysis with three pairs of breast cancer tissues before and after neoadjuvant chemotherapy (NAC) from partially responded patients by Arraystar Human LncRNA Microarray V3.0.

2024-07-16 | GSE221061 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data