Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome characterization of embryogenesis at a single cell resolution [Panagrellus redivivus]

ABSTRACT: By collecting and processing individual embryonic cells from sequentially older embryos we have generated a single cell transcriptome dataset describing embryogenesis from the fertilized zygote and the first cell divisions for all founder cell lines.

ORGANISM(S): Panagrellus redivivus

PROVIDER: GSE86175 | GEO | 2019/08/29

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptome characterization of embryogenesis at a single cell resolution [C. elegans]

Project description:By collecting and processing individual embryonic cells from sequentially older embryos we have generated the first single cell transcriptome dataset describing embryogenesis from the fertilizaed zygote through to the penultimate cell division for most founder cell lines.

2023-03-22 | GSE83523 | GEO

Transcriptome characterization of embryogenesis at a single cell resolution [Acrobeloides nanus]

Project description:By collecting and processing individual embryonic cells from sequentially older embryos we have generated the first comparative single cell transcriptome dataset describing embryogenesis.

2019-08-29 | GSE86192 | GEO

Active Demethylation of Both Maternal and Paternal Genomes in Mouse Zygotes

Project description:The epigenomes of mammalian sperm and oocytes, characterized by gamete-specific 5-methylcytosine (5mC) patterns, are reprogrammed in early embryogenesis to establish full developmental potential. It is broadly accepted that the paternal genome is actively demethylated in the zygote while the maternal genome undergoes passive demethylation thanks to DNA replication over the subsequent cleavage divisions. Here we reveal that both maternal and paternal genomes undergo widespread active and passive demethylation in the pronuclear zygote before the first mitotic division. Whereas the passive demethylation requires DNA replication, the active demethylation relies on enzymatic oxidation of 5mC, as deletion of the DNA dioxygenase, Tet3, but not the inhibition of replication, blocks the active demethylation. At actively demethylated loci, 5mCs appear to be processed to unmodified cytosines in a manner independent of the DNA glycosylase TDG. These observations suggest the occurrence of genuine active demethylation in both parental genomes following fertilization. An extra supportive Tet3 knock-out female pronuclear sample related to experiment Series GSE56650.

2016-09-01 | E-GEOD-60777 | biostudies-arrayexpress

The role of HNRNPU and the nuclear matrix in human pluripotent stem cells [CUT&Tag]

Project description:During early embryogenesis the fertilized zygote proceeds through an intricate developmental program, accompanyed by DNA and chromatin remodelling. However, the mechanisms governing this reconfiguring are poorly understood. Parts of the embryogenesis developmental program can be captured in vitro in the form of two types of human pluripotent stem cells (hPSC): naïve and primed hPSCs. Naïve hPSCs resemble the inner cell mass of the blastocyst, whilst primed hPSCs resemble the post-implantation embryo. The nucleus is made of DNA wrapped around chromatin, and the nuclear matrix, a proteinaceous gel that provides structure. Here, we show that disruptions in the nuclear matrix caused primed hPSCs to spontaneously convert to the naïve, earlier embryonic state.

2024-11-05 | GSE242342 | GEO

The role of HNRNPU and the nuclear matrix in human pluripotent stem cells [ATAC]

2024-11-05 | GSE242341 | GEO

The role of HNRNPU and the nuclear matrix in human pluripotent stem cells [scRNA-Seq]

2024-11-05 | GSE242350 | GEO

The role of HNRNPU and the nuclear matrix in human pluripotent stem cells [RNA-Seq]

2024-11-05 | GSE242348 | GEO

The role of HNRNPU and the nuclear matrix in human pluripotent stem cells [RIP-Seq]

2024-11-05 | GSE242347 | GEO