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Transcriptional Maintenance of Pancreatic Acinar Identity, Differentiation and Homeostasis by PTF1A [6-day RNA-Seq]

ABSTRACT: RNA-seq analysis documented mRNA changes in total pancreatic RNA preparations 6 days after Ptf1a inactivation.

ORGANISM(S): Mus musculus

PROVIDER: GSE86261 | GEO | 2016/09/01

SECONDARY ACCESSION(S): PRJNA341324

REPOSITORIES: GEO

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Transcriptional Maintenance of Pancreatic Acinar Identity, Differentiation and Homeostasis by PTF1A [ChIP-Seq]

Project description:Genome-wide maps of Ptf1a-bound sites in adult pancrea acinar cells.

2016-09-01 | GSE86262 | GEO

Transcriptional Maintenance of Pancreatic Acinar Identity, Differentiation, and Homeostasis by PTF1A.

Project description:Maintenance of cell type identity is crucial for health, yet little is known of the regulation that sustains the long-term stability of differentiated phenotypes. To investigate the roles that key transcriptional regulators play in adult differentiated cells, we examined the effects of depletion of the developmental master regulator PTF1A on the specialized phenotype of the adult pancreatic acinar cell in vivo Transcriptome sequencing and chromatin immunoprecipitation sequencing results showed that PTF1A maintains the expression of genes for all cellular processes dedicated to the production of the secretory digestive enzymes, a highly attuned surveillance of unfolded proteins, and a heightened unfolded protein response (UPR). Control by PTF1A is direct on target genes and indirect through a ten-member transcription factor network. Depletion of PTF1A causes an imbalance that overwhelms the UPR, induces cellular injury, and provokes acinar metaplasia. Compromised cellular identity occurs by derepression of characteristic stomach genes, some of which are also associated with pancreatic ductal cells. The loss of acinar cell homeostasis, differentiation, and identity is directly relevant to the pathologies of pancreatitis and pancreatic adenocarcinoma.

| S-EPMC5126291 | biostudies-literature

Effects on the transcriptome of adult mouse pancreas (principally acinar cells) by the inactivation of the Ptf1a gene in vivo

Project description:RNA-seq analysis documented mRNA changes in total pancreatic RNA preparations 14 days after Ptf1a inactivation.

2015-07-22 | GSE70542 | GEO

Effects on the transcriptome of adult mouse pancreas (principally acinar cells) by the inactivation of the Ptf1a gene in vivo

Project description:RNA-seq analysis documented mRNA changes in total pancreatic RNA preparations 14 days after Ptf1a inactivation. pancreas mRNA profiles of Tamoxifen treated adult control mice [Ptf1a(CreER/+)] and Ptf1a conditional knockout mice [Ptf1a(CreER/fl)] were generated by deep sequencing using an Illumina Hiseq 2500.

2015-07-22 | E-GEOD-70542 | biostudies-arrayexpress

Transcriptional Maintenance of Pancreatic Acinar Identity, Differentiation and Homeostasis by PTF1A

Project description:Transcriptional Maintenance of Pancreatic Acinar Identity, Differentiation and Homeostasis by PTF1A

| PRJNA341322 | ENA

Transcriptional Maintenance of Pancreatic Acinar Identity, Differentiation and Homeostasis by PTF1A

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-09-01 | GSE86263 | GEO

Transcriptional Maintenance of Pancreatic Acinar Identity, Differentiation and Homeostasis by PTF1A [E18.5 RNA-Seq]

Project description:RNA-seq analysis of RNA from embryonic day 18.5 pancreas

2016-09-09 | GSE86568 | GEO

Ptf1a binding sites in a pancreatic cell line which mimics embryonic day E10.5 pancreas progenitors.

Project description:Pancreatic specific transcription factor1a (Ptf1a) immunoprecipitated chromatin from 266.6 cells produced 26 million tags with the Illumina high throughput sequencing technology. Many of the mapped tags were genes which were found to be differentially expressed at E10.5 in the microarray experiments comparing Ptf1a heterozygotes versus null mutant mice (study GSE26816). As Ptf1a is a bHLH transcription factor, it recognizes an E Box on the chromatin CANNTG. Since it belongs to a transcription factor complex, one of which recognizes the binding site TGGGAA, we also found this sequence one, two or three helical turns of DNA away from the E-box where peaks were detected. Ptf1a chromatin immunoprecipitation of cross-linked pancreatic cells, 266.6, to compare with the expressed genes in Ptf1a +/- vs -/- E10.5 pancreatic dorsal buds.

2013-02-14 | E-GEOD-27295 | biostudies-arrayexpress

Ptf1a binding sites in a pancreatic cell line which mimics embryonic day E10.5 pancreas progenitors.

2013-02-14 | GSE27295 | GEO

Comparative gene expression of mouse Pancreatic specific transcription factor 1a (Ptf1a/p48) in pancreatic progenitor cells

Project description:Ptf1a was identified as the essential transcription factor which controls pancreatic exocrine enzyme expression. With lineage tracing eperiments Ptf1a was recognized as an important pancreatic progenitor transcription factor and Ptf1a null mice do not develop a pancreas. We used gene expression arrays to determine the global differeences in expression levels when pancreatic progenitor cells are expanding in Ptf1a heterozygote versus null mutants at E10.5. Ptf1a E10.5 dorsal pancreas total RNA from pools of 3 embryos was twice linear amplified and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 in triplicate for the Ptf1a KO and in duplicate for the Ptf1a heterozygote

2011-01-24 | E-GEOD-26816 | biostudies-arrayexpress

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