Gene expression signatures in whole blood to predict vaccine responses

ABSTRACT: To identify host factors that influence the immunogenicity of the attenuated VZV pOka vaccine strain and the efficacy of VZV vaccination, we immunized 39 individuals aged 50 to 75 years, including 9 monozygotic twin pairs. We measured VZV-specific T cell frequencies by IFN-γ–specific ELISpot, and VZV-specific antibody titers by ELISA. Whole gene expression arrays were performed on vaccinees before (n=28) and one (n=18) or three days (n=10) after vaccination. Cell-specific gene expression profiles were generated by deconvolution using previously described algorithms. Only very few neutrophil- and lymphocyte-related genes changed in expression from day 0 to 1. Significant changes for monocyte-related genes were found, but even here the number of probes with a significant change was low after adjusting for false discovery. When expression changes in monocyte-derived genes were analyzed for their correlation with T cell responses, we identified 493 probes corresponding to 479 genes that correlated with generation of VZV-specific effector T cells and 641 probes corresponding to 621 genes that correlated with the subsequent contraction phase with p<0.05. Interestingly, these two sets of genes were significantly overlapping, i.e., the same changes that were positively or negatively correlated with expansion inversely predicted contraction; their effects therefore cancelled out in determining net benefit in memory cell generation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE86331 | GEO | 2016/09/12

SECONDARY ACCESSION(S): PRJNA341467

REPOSITORIES: GEO