Transcriptomics

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Transcriptome profile in RAW264.7 cells in response to LPS stimulation


ABSTRACT: The lincRNA-Tnfaip3 is an early-primary gene controlled by the NF-κB signaling in murine macrophages. This study aims to measure the impact of lincRNA-Tnfaip3 knockdown on the transcriptome profile in cultured 264.7 macrophages in response to LPS stimulation. Cells were treated with a specific siRNA to knockdown lincRNA-Tnfaip3 and then exposed to LPS for stimulation. Cells treated with a scrambled non-specific siRNA were used as the control. Total RNA was collected for the genome-wide analysis. The Agilent SurePrint G3 Mouse Gene Expression Microarray (G4852A) was used for the genome-wide analysis, which provides full coverage of genes and transcripts with the most up-to-date content, including mRNAs and lincRNAs (http://www.chem.agilent.com/store/en_US/Prod-G4852A/G4852A).

ORGANISM(S): Mus musculus

PROVIDER: GSE86588 | GEO | 2017/10/01

SECONDARY ACCESSION(S): PRJNA342311

REPOSITORIES: GEO

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