Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Analyses of scnRNAs in the mutatnts defective in an RNAi-heterochromatin positive feedback loop in Tetrahymena [scnRNA]

ABSTRACT: In the ciliated protozoan Tetrahymena, an RNAi-mediated feedback loop is important for assembling heterochromatin on the sequences that are removed from the somatic genome by programmed DNA elimination. Because heterochromatin is formed exclusively on the eliminated sequences, some mechanism must inhibit this feedback loop at the boundaries of the eliminated sequences. In this study, we show that the HP1-like protein Coi6p, its interaction partners Coi7p and Lia5p, and the histone demethylase Jmj1p are crucial for confining the production of small RNAs to the eliminated sequences.

ORGANISM(S): Tetrahymena thermophila

PROVIDER: GSE87011 | GEO | 2017/03/06

SECONDARY ACCESSION(S): PRJNA343220

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Analyses of heterochromatin distributions in the mutatnts defective in an RNAi-heterochromatin positive feedback loop in Tetrahymena [ChIP]

Project description:In the ciliated protozoan Tetrahymena, an RNAi-mediated feedback loop is important for assembling heterochromatin on the sequences that are removed from the somatic genome by programmed DNA elimination. Because heterochromatin is formed exclusively on the eliminated sequences, some mechanism must inhibit this feedback loop at the boundaries of the eliminated sequences. In this study, we show that the HP1-like protein Coi6p, its interaction partners Coi7p and Lia5p, and the histone demethylase Jmj1p are crucial for confining the formation of heterochromatin to the eliminated sequences.

2017-03-06 | GSE87013 | GEO

Analyses of Early- and Late-scnRNAs of Tetrahymena

Project description:In the ciliated protozoan Tetrahymena, de novo heterochromatin body formation is accompanied by programmed DNA elimination. We previously reported that dephosphorylation of the HP1-like protein Pdd1p is required for the formation of heterochromatin bodies during the process of programmed DNA elimination in the ciliated protozoan Tetrahymena. Here, we show that the heterochromatin body component Jub4p is required for Pdd1p phosphorylation, heterochromatin body formation and DNA elimination. Moreover, our analyses of unphosphorylatable Pdd1p mutants demonstrate that Pdd1p phosphorylation is required for heterochromatin body formation and DNA elimination, while it is dispensable for local heterochromatin assembly. Therefore, both phosphorylation and the following dephosphorylation of Pdd1p are necessary to facilitate the formation of heterochromatin bodies. We suggest that Jub4p-mediated phosphorylation of Pdd1p creates a chromatin environment that is a prerequisite for subsequent heterochromatin body assembly and DNA elimination. 26 to 32-nt small RNAs from various mutants were analyzed by high-throughput sequencing

2016-07-15 | E-GEOD-83250 | biostudies-arrayexpress

Analyses of Early- and Late-scnRNAs of Tetrahymena

2016-07-15 | GSE83250 | GEO

Analyses of RNAi-heterochromatin positive feedback loop in Tetrahymena

Project description:Analyses of RNAi-heterochromatin positive feedback loop in Tetrahymena

| PRJNA343227 | ENA

Tetrahymena thermophila

Project description:Analyses of heterochromatin distributions in the mutatnts defective in an RNAi-heterochromatin positive feedback loop in Tetrahymena [ChIP]

| PRJNA343283 | ENA

Tetrahymena thermophila

Project description:Analyses of scnRNAs in the mutatnts defective in an RNAi-heterochromatin positive feedback loop in Tetrahymena [scnRNA]

| PRJNA343220 | ENA

Tetrahymena thermophila

Project description:Internal eliminated sequences elimination

| PRJNA416874 | ENA

Twi1p-loaded scnRNAs in GIW1 KO Tetrahymena cells

Project description:To understand the importance of Giw1p in the loading of small RNAs to the Argonaute protein Twi1p in Tetrahymena, we compared Twi1p-bound small RNAs from wild-type cells and GIW1 KO cells. We found that the Twi1p-bound small RNA populations in wild-type cells and GIW1 KO cells are indistinguishable in their size distributions and base profiles.

2014-09-23 | GSE61609 | GEO

EMA2 is required for target-directed scnRNA degradation.

Project description:Piwi-associated small RNAs of the ciliated protozoan Tetrahymena uses such mechanism to induce heterochromatin formation followed by programmed DNA elimination. In this process, target-directed small RNA degradation (TDSD) of small RNAs complementary to the somatic genome enables small RNAs to specifically target the germline-limited sequences for DNA elimination. The data here indicate that the SUMO E3 ligase Ema2 is required for the accumulation of long non-coding RNAs (lncRNAs) from the somatic genome and thus for TDSD.

2023-09-18 | GSE243435 | GEO

Tetrahymena

Project description:Nucleotide sequences of Tetrahymena species

| PRJEB23142 | ENA