Transcriptomics

Dataset Information

0

Control of secreted protein gene expression and the mammalian secretome by the metabolic regulator PGC-1a


ABSTRACT: Secreted proteins serve pivotal roles in the development of multicellular organisms, acting as structural matrix, extracellular enzymes and signal molecules. In this study we demonstrate, unexpectedly, that PGC-1α, a critical transcriptional co-activator of metabolic gene expression, functions to down-regulate expression of diverse genes encoding secreted molecules and extracellular matrix (ECM) components to modulate the secretome. We show that both endogenous and exogenous PGC-1α down-regulate expression of numerous genes encoding secreted molecules. Mechanistically, results obtained using mRNA stability measurements as well as intronic RNA expression analysis are consistent with a transcriptional effect of PGC-1α on expression of genes encoding secreted proteins. Interestingly, PGC-1α requires the central heat shock response regulator HSF1 to affect some of its targets, and both factors co-reside on several target genes encoding secreted molecules in cells. Finally, using a mass spectrometric analysis of secreted proteins, we demonstrate that PGC-1α modulates the secretome of mouse embryonic fibroblasts (MEFs).

ORGANISM(S): Mus musculus

PROVIDER: GSE87100 | GEO | 2016/12/01

SECONDARY ACCESSION(S): PRJNA343540

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2019-06-11 | GSE132458 | GEO
2015-08-07 | E-GEOD-71774 | biostudies-arrayexpress
2023-11-27 | GSE248504 | GEO
2024-05-27 | MSV000094878 | MassIVE
2024-02-05 | GSE241502 | GEO
2024-05-19 | GSE267765 | GEO
2024-02-05 | GSE241503 | GEO
2018-05-22 | GSE110055 | GEO
2018-05-22 | GSE110053 | GEO
2016-04-22 | GSE80521 | GEO