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Real-time quantitative PCR analysis of human acute myeloid leukemia primary cells

ABSTRACT: Acute myeloid leukemia (AML) is a heterogeneous disease characterized by different recurrent cytogenetic and molecular aberrations that occur in hematopoietic progenitor cells and alter the growth, differentiation, and proliferation capacities of the progenitor cells. The treatment of AML has remained a huge challenge for oncohematologists. The nucleoside analog cytarabine, an inhibitor of DNA replication fork progression that results in DNA damage is currently used in the treatment of acute myeloid leukemia. Here, we reasoned that DNA replication represents a less explored source of prognostic markers that could be used to predict AML prognosis and eventually provide potential targets for therapeutic targeting. Accurate execution of the DNA replication program limits cancer risk by preserving genome integrity. Multiple studies of solid cancers have provided evidence that defective or dysregulated DNA replication program triggers replicative stress, leading to the accumulation of genetic alterations. However, defective DNA replication as a source of markers in hematological malignancies has not been explored. Thus we explored the prognostic value of the expression of 72 genes involved in various aspects of DNA replication in a set of 198 AML patients treated by cytarabine-based chemotherapy.

ORGANISM(S): Homo sapiens

PROVIDER: GSE87128 | GEO | 2016/09/21

SECONDARY ACCESSION(S): PRJNA343642

REPOSITORIES: GEO

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