Transcriptomics

Dataset Information

0

In Vitro Derivation and Propagation of Spermatogonial Stem Cell Activity from Mouse Pluripotent Stem Cells


ABSTRACT: The in vitro derivation and propagation of spermatogonial stem cells (SSCs) from pluripotent stem cells (PSCs) is a key goal in reproductive science. We show here that when aggregated with embryonic testicular somatic cells (reconstituted testes), primordial germ cell-like cells (PGCLCs) induced from mouse embryonic stem cells differentiate into spermatogonia-like cells in vitro and are expandable as cells that resemble germline stem cells (GSCs), a primary cell line with SSC activity. Remarkably, GSC-like cells (GSCLCs), but not PGCLCs, colonize adult testes and, albeit less effectively than GSCs, contribute to spermatogenesis and fertile offspring. Whole-genome analyses reveal that GSCLCs exhibit aberrant methylation at vulnerable regulatory elements, including those critical for spermatogenesis, which may restrain their spermatogenic potential. Our study establishes a strategy for the in vitro derivation of SSC activity from PSCs, which, we propose, relies on faithful epigenomic regulation.

ORGANISM(S): Mus musculus

PROVIDER: GSE87341 | GEO | 2016/12/06

SECONDARY ACCESSION(S): PRJNA344462

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2016-12-06 | GSE76245 | GEO
2021-09-30 | GSE168220 | GEO
2021-09-30 | GSE168221 | GEO
2022-03-05 | GSE178701 | GEO
2022-12-31 | GSE189805 | GEO
2021-12-31 | GSE139432 | GEO
| phs001777 | dbGaP
2020-08-20 | GSE156505 | GEO
2021-07-15 | GSE158190 | GEO
2021-12-10 | PXD023802 | Pride