Transcriptomics

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Sequential responses to high-fat feeding in an obese mouse model


ABSTRACT: We examined the effects of high-fat diet on feeding behaviour, body weight regulation and common biomarkers associated with weight gain in the C57BL/6J mice over a period of 10 weeks, making measurements at weeks 2, 4 and 10. We examined the transcriptomic profile of hepatic genes involved in the major lipid metabolic pathways, validating the key genes with quantitative real-time reverse-transcription PCR (qRT-PCR) and their gene products with western blots. Keywords: Lipogenesis, Hyperphagia, Fat Oxidation, Energy Regulation, Body Weight

ORGANISM(S): Mus musculus

PROVIDER: GSE8753 | GEO | 2007/12/01

SECONDARY ACCESSION(S): PRJNA102023

REPOSITORIES: GEO

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