Transcriptomics

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MYCN-transformed neuroblasts from TH-MYCN mice are selectively isolated and maintained as spheres in vitro


ABSTRACT: It has been already reported that there are undifferentiated/proliferating neuroblasts in the postnatal sympathetic ganglia in TH-MYCN mice. We established suitable spheroid culture condition that selectively isolates undifferentiated neuroblasts from superior mesenteric ganligon (SMG) of TH-MYCN mice. In the present study, in order to investigate the transcriptomic differences among 3-week-old WT SMG, TH-MYCN (hemizygote) SMG, and TH-MYCN (hemizygote) spheres, we carried out microarray gene expression analysis. We investigated whether or not undifferentiated cells observed in TH-MYCN SMG were selectively isolated and maintaned as spheres.

ORGANISM(S): Mus musculus

PROVIDER: GSE87782 | GEO | 2017/04/01

SECONDARY ACCESSION(S): PRJNA347592

REPOSITORIES: GEO

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