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Mapping cell type-specific transcriptional enhancers using high affinity, lineage-specific p300 bioChIP-seq

ABSTRACT: We report a method for high throughput mapping of transcriptional enhancers, genomic regulatory regions that activate lineage-specific transcription. Since tissue-specific enhancers are bound by the transcriptional co-activator p300, we developed murine knock-in alleles that permit Cre-dependent, lineage-specific p300 in vivo biotinylation and high affinity pulldown on immobilized streptavidin. Subsequent next generation sequencing of p300-bound genomic DNA identified lineage-specific enhancers. By driving this system with lineage-specific Cre transgenes, we mapped enhancers active in embryonic endothelial cells/blood or skeletal muscle. Analysis of these enhancer sequences identified new transcription factor heterodimer motifs that likely regulate transcription in these lineages. Furthermore, we identified candidate enhancers that regulate adult heart- or lung- specific endothelial cell specialization.

ORGANISM(S): Mus musculus

PROVIDER: GSE88789 | GEO | 2017/01/24

SECONDARY ACCESSION(S): PRJNA348551

REPOSITORIES: GEO

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ChIP-seq Accurately Predicts Tissue-Specific Activity of Enhancers

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Mus musculus

Project description:Mapping cell type-specific transcriptional enhancers using high affinity, lineage-specific p300 bioChIP-seq

| PRJNA348551 | ENA

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