Transcriptomics

Dataset Information

0

Two MicroRNAs and NF-kB Act in a Unique Regulatory Network to Ensure Precision of the Acute Inflammatory Response in Macrophages


ABSTRACT: The innate inflammatory response must be tightly regulated to ensure effective immune protection while avoiding inflammation-related pathologies. The transcription factor NF-kB is a critical mediator of the inflammatory response, and its dysregulation has been associated with immune related malignancies. We herein show that miR-155, miR-146a and NF-kB form a regulatory network that tunes the macrophage inflammatory response in mice. We show that elevated miR-155 expression potentiates NF-kB activity in miR-146a deficient mice, thus leading to an overactive acute inflammatory response and chronic inflammation. Enforced miR-155 expression overrides miR-146a-mediated repression of NF-kB activation, thus emphasizing that miR-155 plays a dominant, downstream role in promoting inflammation. We further show that miR-155 deficient macrophages exhibit a suboptimal inflammatory response when exposed to low levels of inflammatory stimuli. Importantly, we demonstrate a temporal asymmetry between miR-155 and miR-146a expression during macrophage activation, which forms a combined positive and negative feedback network on NF-kB activity. This miRNA based regulatory network enables a robust and time-limited inflammatory response essential for functional immunity.

ORGANISM(S): Mus musculus

PROVIDER: GSE88791 | GEO | 2017/07/18

SECONDARY ACCESSION(S): PRJNA348555

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2013-08-01 | GSE42506 | GEO
2013-08-01 | E-GEOD-42506 | biostudies-arrayexpress
2014-06-30 | E-GEOD-52783 | biostudies-arrayexpress
2020-01-31 | GSE138222 | GEO
2020-05-14 | GSE148989 | GEO
2020-05-14 | GSE148988 | GEO
2016-07-15 | E-GEOD-77425 | biostudies-arrayexpress
2014-06-30 | GSE52783 | GEO
2020-03-30 | GSE122940 | GEO
2020-03-30 | GSE134161 | GEO