Genetic circuit 0x58
Ontology highlight
ABSTRACT:
ORGANISM(S): Escherichia coli
PROVIDER: GSE88835 | GEO | 2017/09/29
SECONDARY ACCESSION(S): PRJNA348749
REPOSITORIES: GEO
Similar Datasets
Project description:We report the characterization of the 0x58 circuit, a modified version and wild-type cells not containing any circuit. Data is collected for all input inducer combinations and cells harboring the circuit are grown in culture tubes.
2017-09-29 | GSE98890 | GEO
Project description:For the microarray experiments, 10 g glass wool (Corning Glass Works, Corning, N.Y.) were used to form biofilms (30) in 250 mL in 1 L Erlenmeyer shake flasks which were inoculated with overnight cultures diluted that were 1:100. For EHEC with 7-hydroxyindole and isatin, 1000 mM 7-hydroxyindole in 250 mL DMF, 250 mM isatin in 250 mL DMF, or 250 mL DMF alone were added to cells grown in LB. The cells were shaken at 250 rpm and 30°C for 7 hours to form biofilms on the glass wool, and RNA was isolated from the suspension cells and the biofilm. Keywords: Biofilm study
2007-04-26 | GSE6195 | GEO
Project description:For the microarray experiments, 10 g glass wool (Corning Glass Works, Corning, N.Y.) were used to form biofilms (30) in 250 mL in 1 L Erlenmeyer shake flasks which were inoculated with overnight cultures diluted that were 1:167. The cells were shaken at 250 rpm and 37°C for 24 hours to form biofilms on the glass wool, and RNA was isolated from the suspension cells and the biofilm. Keywords: E. coli biofilm
2007-09-21 | GSE6925 | GEO
Project description:We used DNA microarrays to investigate the impact of indole and 7-hdroxyindole on P. aeruginosa PAO1.For the microarray experiments, 10 g glass wool (Corning Glass Works, Corning, N.Y.) were used to form biofilms in 250 mL in 1 L Erlenmeyer shake flasks which were inoculated with overnight cultures of P. aeruginosa PAO1 diluted that were 1:100. For P. aeruginosa with 7-hydroxyindole and indole, 500 uM 7-hydroxyindole in 250 uL DMF, 1000 uM indole in 250 uL DMF, or 250 uL DMF alone were added to cells grown in LB. The cells were shaken at 250 rpm and at 37oC for 7 h to form biofilms on the glass wool, and RNA was isolated from the biofilm cells. Keywords: comparison with E. coli signal indole on P. aeruginosa PAO1
2008-11-21 | GSE10065 | GEO
Project description:We used DNA microarrays to investigate the impact of indole and 7-hdroxyindole on P. aeruginosa PAO1.For the microarray experiments, 10 g glass wool (Corning Glass Works, Corning, N.Y.) were used to form biofilms in 250 mL in 1 L Erlenmeyer shake flasks which were inoculated with overnight cultures of P. aeruginosa PAO1 diluted that were 1:100. For P. aeruginosa with 7-hydroxyindole and indole, 500 uM 7-hydroxyindole in 250 uL DMF, 1000 uM indole in 250 uL DMF, or 250 uL DMF alone were added to cells grown in LB. The cells were shaken at 250 rpm and at 37oC for 7 h to form biofilms on the glass wool, and RNA was isolated from the biofilm cells. Keywords: comparison with E. coli signal indole on P. aeruginosa PAO1 For the microarray experiments, 10 g glass wool (Corning Glass Works, Corning, N.Y.) were used to form biofilms in 250 mL in 1 L Erlenmeyer shake flasks which were inoculated with overnight cultures of P. aeruginosa PAO1 diluted that were 1:100. For P. aeruginosa with 7-hydroxyindole and indole, 500 uM 7-hydroxyindole in 250 uL DMF, 1000 uM indole in 250 uL DMF, or 250 uL DMF alone were added to cells grown in LB. The cells were shaken at 250 rpm and at 37oC for 7 h to form biofilms on the glass wool, and RNA was isolated from the biofilm cells.
2010-10-23 | E-GEOD-10065 | biostudies-arrayexpress
Project description:For the microarray experiments, 10 g glass wool (Corning Glass Works, Corning, N.Y.) were used to form biofilms (30) in 250 mL in 1 L Erlenmeyer shake flasks which were inoculated with overnight cultures diluted that were 1:100. For EHEC with 7-hydroxyindole and isatin, 1000 mM 7-hydroxyindole in 250 mL DMF, 250 mM isatin in 250 mL DMF, or 250 mL DMF alone were added to cells grown in LB. The cells were shaken at 250 rpm and 30°C for 7 hours to form biofilms on the glass wool, and RNA was isolated from the suspension cells and the biofilm. Experiment Overall Design: Strain: EHEC EDL933 (ATCC 43895) wild type Experiment Overall Design: Medium: LB Experiment Overall Design: Cells: Suspension cells and biofilm cells on glass wool Experiment Overall Design: Time: 7h
2008-06-14 | E-GEOD-6195 | biostudies-arrayexpress
Project description:Gene expression profiles of Bacillus subtilis strain AG174 were compared as a function of steady-state external pH during growth with aeration. Aerated overnight cultures were diluted 1:500 and incubated in 250 mL baffled flasks containing potassium-modified Luria-Bertani medium (LBK) buffered with 50 mM HOMOPIPES at pH 6.0, pH 7.0, and pH 9.0. Tubes were incubated at 37°C with aeration (260 rpm) to an optical density at 600 nm of 0.2. For each of the three pH conditions, RNA was isolated from five independent cultures.
2009-01-13 | GSE11810 | GEO
Project description:For the microarray experiments, 10 g glass wool (Corning Glass Works, Corning, N.Y.) were used to form biofilms (30) in 250 mL in 1 L Erlenmeyer shake flasks which were inoculated with overnight cultures diluted that were 1:167. The cells were shaken at 250 rpm and 37°C for 24 hours to form biofilms on the glass wool, and RNA was isolated from the suspension cells and the biofilm. Experiment Overall Design: Strain: E. coli BW25113 wild type and ymgB mutant Experiment Overall Design: Medium: LBglu Experiment Overall Design: Cells: biofilm cells on glass wool Experiment Overall Design: Time: 24h
2008-06-15 | E-GEOD-6925 | biostudies-arrayexpress
Project description:Gene expression profiles of Bacillus subtilis strain AG174 were compared as a function of steady-state external pH during growth with aeration. Aerated overnight cultures were diluted 1:500 and incubated in 250 mL baffled flasks containing potassium-modified Luria-Bertani medium (LBK) buffered with 50 mM HOMOPIPES at pH 6.0, pH 7.0, and pH 9.0. Tubes were incubated at 37°C with aeration (260 rpm) to an optical density at 600 nm of 0.2. For each of the three pH conditions, RNA was isolated from five independent cultures. Overnight cultures were diluted 1:500 and incubated in 250 mL baffled flasks containing potassium-modified Luria-Bertani medium (LBK) buffered with 50 mM HOMOPIPES at pH 6.0, pH 7.0, and pH 9.0. Cultures were incubated at 37°C with aeration (260 rpm) to an optical density at 600 nm of 0.2. Five replicate cultures were grown at each pH.
2009-01-12 | E-GEOD-11810 | biostudies-arrayexpress
Project description:The influence of substrate composition on extracellular protein production was evaluated in media containing glucose, lodgepole pine or aspen as carbon sources. Common to all media, basal salts contained, per liter, 2g NH4NO3, 2g KH2, 0.5 g MgSO4·7H2O, 0.1 g CaCl2·2H2O, 1 mg thiamine hydrochloride and 1 mL trace element solution (Teknova, T1001). Aspen and pine were added to 5 g per liter, while the glucose cultures received 2.5 g per liter. All cultures contained 100 mL media in 500 ml Erlenmeyer flasks and, following inoculation with macerated mycelium, incubated on a rotary shaker (100 RPM) at 24°C. Aspen (Populus grandidentata) and lodgepole pine (Pinus contorta) were Wiley milled and sieved to one mm mesh prior to inoculation. Triplicate cultures were harvested after 5 and 10 days.
2023-09-15 | PXD045113 | Pride