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Impact of hypothermic preservation of mouse kidney resident immune cells

ABSTRACT: With the growing interest in studying primary tissue samples by single cell transcriptome analysis, there is an emerging demand for a preservation strategy that enables sample transportation and storage. In this study, we describe a simple and general strategy that preserves primary tissues at hypothermic temperature. Using FACS and single-cell RNAseq, we demonstrated the effectiveness of this strategy in maintaining cell viability, cell population heterogeneity, and cell transcriptome integrity for primary tissues that underwent up to 3 days of preservation.

ORGANISM(S): Mus musculus

PROVIDER: GSE88953 | GEO | 2018/02/21

REPOSITORIES: GEO

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Mus musculus

Project description:Impact of hypothermic preservation of mouse kidney resident immune cells

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