Study on the effects of cytoskeletal modifying compounds on stem cell differentiation
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ABSTRACT: We report genome-wide expression changes that occur in mouse bone marrow-derived mesenchymal stem cells treated in triplicate for 24 hours with or without Cytochalasin D and/or CK666. mRNA-Seq analysis shows that both cell surface and the nucleus undergo phenotypic changes. Cytochalasin D enhanced expression of genes involved in pathways known to regulate osteoblast differentiation, including genes involved in development and cell signaling, including calcium ion binding, WNT and PI3K/AKT pathway. In summary, RNA-seq data reveal that the CytoD activates genes linked to osteogenesis, while CK666stimulates adipogenic genes.
ORGANISM(S): Mus musculus
PROVIDER: GSE89132 | GEO | 2016/12/30
SECONDARY ACCESSION(S): PRJNA350338
REPOSITORIES: GEO
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