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Easy Hi-C: A simple efficient protocol for 3D genome mapping in small cell populations


ABSTRACT: Despite the growing interest in studying the mammalian genome organization, it is still challenging to map the DNA contacts genome-wide. Here we present easy Hi-C (eHi-C), a highly efficient method for unbiased mapping of 3D genome architecture. The eHi-C protocol only involves a series of enzymatic reactions and maximizes the recovery of DNA products from proximity ligation. We show that eHi-C can be performed with 0.1 million cells and yields high quality libraries comparable to Hi-C.

ORGANISM(S): Homo sapiens

PROVIDER: GSE89324 | GEO | 2017/10/31

SECONDARY ACCESSION(S): PRJNA351790

REPOSITORIES: GEO

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