Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of loss of CREB and CREM on kainate-induced gene expression in the hippocampus (MG-U74A).

ABSTRACT: Ablation of the Creb1 gene in forebrain neurons was performed using the Cre/loxP system, with the recombinase expressed from the Camk2alfa promoter. Mice were crossed into the Crem KO background to prevent compensation of CREB loss by CREM overexpression. Our goal was to analyze how loss of CREB will affect acitivity-regulated transcription induced by strong stimulation, i.e. kainate. Keywords: Treatment x Genotype

ORGANISM(S): Mus musculus

PROVIDER: GSE8944 | GEO | 2008/04/03

SECONDARY ACCESSION(S): PRJNA102369

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Effect of loss of CREB and CREM on kainate-induced gene expression in the hippocampus (MG-U74C).

2008-04-03 | GSE8947 | GEO

Effect of loss of CREB and CREM on kainate-induced gene expression in the hippocampus (MG-U74B).

2008-04-03 | GSE8946 | GEO

Transcription profiling of mouse hippocampus with loss of CREB and CREM after kainate-induced gene expression in the hippocampus

Project description:Ablation of the Creb1 gene in forebrain neurons was performed using the Cre/loxP system, with the recombinase expressed from the Camk2alfa promoter. Mice were crossed into the Crem KO background to prevent compensation of CREB loss by CREM overexpression. Our goal was to analyze how loss of CREB will affect acitivity-regulated transcription induced by strong stimulation, i.e. kainate. Experiment Overall Design: Animals were injected i.p. with either 20 mg/kg kainate or saline and sacrificed after one hour by cervical dislocation. Expression profiling was performed using total RNA isolated from the hippocampus.

2008-06-16 | E-GEOD-8947 | biostudies-arrayexpress

Transcription profiling of mouse Creb1 abalated hippocampus fin a Crem knockout background treated with kainate

2008-06-16 | E-GEOD-8946 | biostudies-arrayexpress

Effect of loss of CREB and CREM on cocaine-induced gene expression in the striatum

2008-04-03 | GSE8948 | GEO

Transcription profiling of mouse hippocampus from animals with Creb1 abalated forebrains in a Crem1 knockout background

2008-06-16 | E-GEOD-8944 | biostudies-arrayexpress

Myometrial cells expressing CREB, CREM alpha, CREM tau2alpha, ATF2 or the ATF2-small gene

Project description:Human myometrial cells taken from biopsies of pre-menopausal non-cancerous non-pregnant uteri were cultured in complete MEM D-valine medium plus 10% FCS, and stably-transfected with the pcDNA3.1/V5-His TOPO vector (Invitrogen) harbouring either CREB, CREM alpha, CREM tau2alpha, ATF2, ATF2-small gene or a control empty-vector. Total RNA was extracted using TRI-reagent (Sigma) and RNeasy columns (Qiagen), and 10 ug used in target preparation for array hybridization. Keywords: other

2004-02-21 | GSE1059 | GEO

Transcription profiling of human myometrial cells expressing CREB, CREM alpha, CREM tau2alpha, ATF2 or the ATF2-small gene

2007-07-19 | E-GEOD-1059 | biostudies-arrayexpress

The effect of Crem absence on gene expression in mouse testis.

Project description:CREM (cAMP responsive element modulator) together with CREB and ATF-1 belong to the CREB family of transcriptional factors, that respond to cyclic AMP signaling and bind to cAMP responsive element (CRE) sites in promoters of selected genes. CREM can produce isoforms that have either activating or repressing functions, depending on the transcription of specific exons. In testis, it is involved in the regulation of spermatogenesis. In this dataset, we include the expression data obtained from wild-type and Crem knock-out mouse testis. 2 condition experiment: 2 strains (WT, Crem-/-). 5 biological replications per condition.

2012-04-10 | E-GEOD-29593 | biostudies-arrayexpress

The effect of Crem absence on gene expression in mouse testis.

2012-04-11 | GSE29593 | GEO