Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Impact of biotic and abiotic factors on the expression of fungal effector-encoding genes in axenic growth conditions

ABSTRACT: In phytopathogenic fungi, the expression of hundreds of small secreted protein (SSP)-encoding genes is induced upon primary infection of plants while no or a low level of expression is observed during vegetative growth. In some species such as Leptosphaeria maculans, this coordinated in-planta upregulation of SSP-encoding genes expression relies on an epigenetic control but the signals triggering gene expression in-planta are unknown. In the present study, biotic and abiotic factors that may relieve suppression of SSP-encoding gene expression during axenic growth of L. maculans were investigated. Some abiotic factors (temperature, pH) could have a limited effect on SSP gene expression. In contrast, two types of cellular stresses induced by antibiotics (cycloheximide, phleomycin) activated strongly the transcription of SSP genes. A transcriptomic analysis to cycloheximide exposure revealed that biological processes such as ribosome biosynthesis and rRNA processing were induced whereas important metabolic pathways such as glycogen and nitrogen metabolism, glycolysis and tricarboxylic acid cycle activity were down-regulated. A quantitatively different expression of SSP-encoding genes compared to plant infection was also detected. Interestingly, the same physico-chemical parameters as those identified here for L. maculans effectors were identified to regulate positively or negatively the expression of bacterial effectors. This suggests that apoplastic phytopathogens may react to similar physiological parameters for regulation of their effector genes.

ORGANISM(S): Plenodomus lingam

PROVIDER: GSE89558 | GEO | 2019/11/04

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Leptosphaeria maculans gene expression during in vitro growth after silencing of DIM5 or HP1, two genes involved in chromatin remodelling

2014-03-06 | GSE50616 | GEO

Leptosphaeria maculans gene expression during in vitro growth after silencing of DIM5 or HP1, two genes involved in chromatin remodelling

Project description:The transcriptome of Leptosphaeria maculans was analysed in mycelium of the wild type isolate v23.1.3 or in transformants silenced for DIM5 or HP1, two genes encoding enzymes involved in chromatin remodelling. The array probes were designed from gene models from the L. maculans whole genome annotation. The aim of this study was to characterise the effect of chromatin remodelling on gene expression during in vitro growth. We performed 9 hybridizations (NimbleGen) with samples derived from mycelium of a wild type isolate, v23.1.3, of a transformant silenced for HP1 and for a transformant silenced for DIM5. Three replicates each. All samples were labeled with Cy3.

2014-03-06 | E-GEOD-50616 | biostudies-arrayexpress

Regulation of effector gene expression as concerted waves in Leptosphaeria maculans: a two-players game

Project description:During infection, plant pathogenic fungi secrete a set of molecules collectively known as effectors, involved in overcoming the host immune defense system and in disease establishment. Effector genes are concertedly expressed as waves all along plant pathogenic fungi lifecycle. However, little is known about how coordinated expression of effector genes is regulated. Since many effector genes are located in repeat-rich regions, the role of chromatin remodeling in the regulation of effector expression was recently investigated. In Leptosphaeria maculans, causing stem canker of oilseed rape, we established that the repressive histone modification H3K9me3 (trimethylation of Lysine 9 of Histone H3), deposited by the histone methyltransferase KMT1, was involved in the regulation of expression of genes highly expressed during infection, including effectors. Nevertheless, inactivation of KMT1 did not induce expression of these genes at the same level as observed during infection of oilseed rape, suggesting that a second regulator, such as a transcription factor (TF), might be involved. Pf2, a TF belonging to the Zn2Cys6 fungal specific TF family, was described in several Dothideomycete species as essential for pathogenicity and effector gene expression. We identified the orthologue of Pf2 in L. maculans, LmPf2, and investigated the role of LmPf2 together with KMT1 in L. maculans, by inactivating and over-expressing LmPf2 in a wild type (WT) strain and a ∆kmt1 mutant. Functional analyses of the corresponding transformants highlighted an essential role of LmPf2 in the establishment of pathogenesis. Transcriptomic analyses during axenic growth showed that LmPf2 is involved in the control of effector gene expression. We observed an enhanced effect of the over-expression of LmPf2 on effector gene expression in a ∆kmt1 background, suggesting a synergic role between KMT1 and LmPf2.

2024-01-01 | GSE236510 | GEO

Gene expression profile in MS medium for the phytopathogenic fungus Leptosphaeria maculans brassicae (Lmb) and lepidii (Lml)

Project description:Full transcriptomes of Leptosphaeria maculans ‘brassicae’ v23.1.3 and Leptosphaeria maculans ‘lepidii’ IBCN84, grown in Fries medium, were studied.

2021-06-02 | GSE150126 | GEO

Gene expression comparison of P. atrosepticum and it's derivative vasH mutant

Project description:Effect of mutagenesis of a sigma 54-dependent transcriptional regulator encoding gene vasH (ECA3425) to global gene expression in P. atrosepticum SCRI1043 in planta mimicking conditions.

2013-07-03 | GSE48471 | GEO

Host transcriptional reprogramming in response to the rice bacterial blight pathogen Xanthomonas oryzae pv. oryzae strain BLS354

Project description:We performed RNA-Seq of leaves of Oryza sativa L. ssp. japonica cv. Nipponbare 48 hours after inoculation with Xanthomonas oryzae pv. oryzicola strain BLS354, the causal agent of bacterial leaf streak. Results provide insight into the molecular basis of bacterial leaf streak, particularly the role of transcription activator-like effectors in the disease. Examination of mRNA levels in Oryza sativa L. ssp. japonica cv. Nipponbare leaves at 48 hours after inoculation with Xanthomonas oryzae pv. oryzicola strain BLS354 with three biological replicates compared to three replicates of mock inoculated O. sativa as the control.

2016-07-07 | E-GEOD-75131 | biostudies-arrayexpress

Time series transcriptome of Venturia inaequalis (apple scab) infecting Malus domestica (apple)

Project description:V. inaequalis causes apple scab disease, the most economically important disease of apples. In this study, we generated a comprehensive RNA-seq transcriptome of V. inaequalis during host colonization of apple, with six in planta time points (12hpi, 24hpi, 2dpi, 3dpi, 5dpi, 7dpi) and one in culture reference (fungus grown on cellophane membranes overlaying potato dextrose agar). Analysis of this transcriptome identified five in planta gene expression clusters or waves corresponding to three specific infection stages: early, mid and mid-late infection of subcuticular biotrophic host-colonization. In our analysis we focus on general fungal nutrition (plant cell wall degrading enzymes and transporters) as well as effectors (proteinaceous effectors and secondary metabolites). Early infection was characterized by the expression of genes that encode plant cell wall-degrading enzymes (PCWDEs) and proteins associated with oxidative stress responses. Mid-late infection was characterized by genes that encode PCWDEs and effector candidates (ECs).

2022-03-14 | GSE198244 | GEO

Host transcriptional reprogramming across diverse strains of the rice bacterial leaf streak pathogen Xanthomonas oryzae pv.oryzicola

Project description:We performed RNA-Seq of leaves of Oryza sativa L. ssp. japonica cv. Nipponbare 48 hours after inoculation with 10 geographically diverse strains of Xanthomonas oryzae pv. oryzicola, the causal agent of bacterial leaf streak. Results provide insight into the molecular basis of bacterial leaf streak, particularly the role of transcription activator-like effectors in the disease. Examination of mRNA levels in Oryza sativa L. ssp. japonica cv. Nipponbare leaves at 48 hours after inoculation with 10 strains of Xanthomonas oryzae pv.oryzicola with three biological replicates for each compared to three replicates of mock inoculated O sativa as the control

2015-05-01 | E-GEOD-67588 | biostudies-arrayexpress

Host transcriptional reprogramming in response to the rice bacterial blight pathogen Xanthomonas oryzae pv. oryzae strain BLS354

2016-07-07 | GSE75131 | GEO

Pseudomonas syringae effector HopZ3 suppresses PTO immune pathway in tomato by acetylation

Project description:The plant pathogen Pseudomonas syringae secretes multiple effectors to suppress plant defenses. Some effectors are recognized by plants while others can function to suppress the resulting immune responses. HopZ3 of Psy B728a is an aminotransferase targeting multiple components of both plant immune complexes and Psy effectors, leading to the activation of these complexes. In Arabidopsis, HopZ3 acetylates the host RPM1 complex and Psy effectors AvrRpm1 and AvrB3 s. In P. syringae resistant tomato, the main immune complex includes PRF and PTO. We found that HopZ3 acts as a virulence factor on tomato by suppressing defenses triggered by Psy AvrPto1. HopZ3 interacts with members of the PTO complex and acetylates PTO, AvrPto1, SlRIPK and SlRIN4s in vitro and in planta. Mapping HopZ3-dependent acetylation sites in planta revealed that AvrPto1 is modified on histidine, serine and threonine residues. The most highly acetylated AvrPto1 residues are T91 and S94 in a loop essential for interaction with PTO. HopZ3 also acetylates, in planta, T204 and T199 in the PTO activation loop which is important for kinase activity and recognition of AvrPto1. In agreement with these findings, we showed that acetylation of either AvrPto1 or PTO by HopZ3 reduces their binding in vitro. Mutation of acetylation sites T91A or H125A/H135A in AvrPto1 does not affect PTO binding in vitro but decreases AvrPto1 recognition in tomato in the absence of HopZ3 and increases bacterial growth. We also found decreased in planta phosphorylation in the presence of HopZ3 for some residues in AvrPto1 and PTO involved in immune signaling. Moreover, acetylation of SlRIPK by HopZ3 reduces its activity as well as phosphorylation of HopZ3 and SlRIN4s. Psy modulation of both its own avirulence effectors and their plant targets leads to decreased defenses and increased susceptibility to infection.

2021-10-18 | PXD022953 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data