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Stepwise chromatin remodeling by two lincRNAs underlies cell fate in fission yeast

ABSTRACT: The cell-fate decision leading to gametogenesis requires the convergence of multiple signals on the promoter of a master regulator. In fission yeast, starvation-induced signaling leads to the transcriptional induction of the ste11 gene, which enodes the central inducer of mating and gametogenesis, know as sporulation. We find that the long intergenic non-coding (linc) RNA rse1 is transcribed divergently upstream of the ste11 gene. rse1 directly recruits a Mug187-Lid2-Set1 complex that mediates SET3C-dependent deacetylation and repression at the ste11 promoter. Upon starvation, the activation of the ste11 promoter is bidirectional, generating a second lincRNA, rce1, overlapping the promoter of rse1. The transcription of rce1 establishes repressive chromatin at the rse1 promoter, therefore relieving the repression on ste11. Thus, the transcription of two lincRNAs governs the switch from vegetative growth to sexual differentiation in fission yeast. Our data reveals that the remodeling of chromatin through ncRNA scaffolding of repressive complexes that is observed in higher eukaryotes is a conserved, likely very ancient mechanism for tight control of cell differeniation.

ORGANISM(S): Schizosaccharomyces pombe

PROVIDER: GSE89825 | GEO | 2019/11/13

REPOSITORIES: GEO

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