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Next-Generation Sequencing Facilitates Quantitative Analysis of Wild Type and abi5 Transcriptomes

ABSTRACT: It has been known that ABA INSENSITIVE 5 (ABI5) plays a vital role in regulating seed germination. In the present experiment, we showed that 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of the loss-of-function mutant abi5-1, but promotes seed germination of the ABI5-overexpression transgenic line, and that ABI5 affects reactive oxygen species (ROS) homeostasis. RNA-sequencing analysis indicates that the transcription of the genes involved in ROS metabolic process or genes responsive to ROS stress is impaired in abi5-1 seeds. Together, all the findings suggest that ABI5 regulates seed germination at least partly by affecting ROS homeostasis.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE90004 | GEO | 2017/07/11

SECONDARY ACCESSION(S): PRJNA354330

REPOSITORIES: GEO

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