Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

High throughput sequencing in synchronized yeast meiotic culture with pCUP1-IME1 pCUP1-IME4 strain

ABSTRACT: We used pCUP1-IME1 pCUP1-IME4 strain to generate synchronized yeast meiotic culture and measure the RNA expression through meiotic progression

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE90008 | GEO | 2016/11/18

SECONDARY ACCESSION(S): PRJNA354531

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Synchronized yeast meiotic culture timecourse

Project description:Synchronized yeast meiotic culture timecourse

| PRJNA354329 | ENA

Yeast meiotic transcriptional profile

Project description:RNAseq across a time cours of meiosis of cells with and without Kar4p. The overexpression of either IME1 or both IME1 and RIM4 was used to determine how overexpression of the two genes functions to resuce the meiotic defect associated with the loss of Kar4p.

2023-02-01 | GSE221451 | GEO

Yeast meiotic transcriptional profile [Kfc1p mutant]

Project description:RNAseq across a time course of meiosis of cells with and without Kfc1p. The overexpression of either IME1 or both IME1 and RIM4 was used to determine how overexpression of the two genes functions to resuce the meiotic defect associated with the loss of Kar4p.

2023-02-01 | GSE222684 | GEO

Yeast meiotic transcriptom kar4 delete v. wild type

Project description:Transcriptional profiling of wild type and kar4 deletion yeast mutants across a meiotic time course. Including samples with IME1 and RIM4 overexpression

2023-02-01 | GSE220125 | GEO

Gene expression profiles of WT and ime4-/- mutant yeast cells, under vegetative and meiosis-inducing conditions

Project description:Inactivation of the yeast IME4 gene, the yeast homologue of METTL3, was shown to result in the loss of m6A in mRNA of mutant cells grown in sporulation medium. We attempted to characterize the effects of ime4 deletion on gene expression under vegetative and meiosis-inducing conditions. The results show that in vegetatively-growing ime4-/- cells there is an increased expression of the RME1 gene (repressor of meiosis) which prevents precocious entry into the meiotic program. Mutant yeast cells showed reduced expression levels of genes involved in ribosome biogenesis and gene expression processes. Surprisingly, despite the fact that a diploid strain was analyzed, there was also a striking change in the expression level of haploid-specific genes, suggesting that RNA methylation may be used to enforce the sexual identity of diploid cells, required for the implementation of the gametogenesis program. Consistently, when cells were induced to undergo meiosis, ime4-/- diploids failed to undergo the meiotic divisions. Among the genes showing reduced expression in the mutant were IME1 and IME2, the two known inducers of meiosis. Thus, the yeast IME4 gene plays an important role in the regulation of the developmental switch from vegetative cells into gametogenesis. WT and ime4 -/- yeast cells were grown vegetatively in YPD (Yeast extract, Peptone, Dextrose) medium, and transferred to sporulation medium(1% Kacetate) for induction of sporulation for 4 hrs. RNA was purified from the cells and hybridized to Affymetrix microarrays. Experiments were conducted in biological replicates.

2012-04-24 | E-GEOD-36958 | biostudies-arrayexpress

Saccharomyces cerevisiae

Project description:High throughput sequencing in synchronized yeast meiotic culture with pCUP1-IME1 pCUP1-IME4 strain

| PRJNA354531 | ENA

Gene expression profiles of WT and ime4-/- mutant yeast cells, under vegetative and meiosis-inducing conditions

2012-04-25 | GSE36958 | GEO

Synchronized yeast meiotic culture timecourse

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-11-18 | GSE90009 | GEO

Yeast (Saccharomyces cerevisiae) synchronized meiotic timecourse II

Project description:transcriptional profiling of yeast meiotic culture

2016-11-18 | GSE90006 | GEO