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A novel single-strands RNA virus in Nesidiocoris tenuis

ABSTRACT: RNA was isolated from adults using the TRIzol (Invitrogen) reagent by following the company manual. Poly(A) mRNA was isolated from 20 µl total RNA using Oligo (dT) magnetic beads and then was broken into short fragments (about 200bp) in the presence of fragmentation buffer at 94 °C for 5 min. The mRNA samples were used to construct the cDNA libray with mRNA-Seq assay.The libraries were sequenced using HiSeq4000 (Illumina) in paired-end mode, creating reads with a length of 150 bp.

ORGANISM(S): Nesidiocoris tenuis

PROVIDER: GSE90671 | GEO | 2018/11/27

REPOSITORIES: GEO

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