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Tethered Oligonucleotide-Primed sequencing (TOP-seq): a high resolution economical approach for DNA epigenome profiling

ABSTRACT: We describe the development and thorough validation of a new approach for epigenome analysis (named TOP-seq) that is based on a novel concept – combination of selective covalent tagging and targeted genomic sequencing of the genome. The technique reports the modification status of each genomic CpG site by priming the DNA polymerase action from a tethered oligonucleotide probe that is selectively attached to the unmodified CpG sites.

ORGANISM(S): Homo sapiens

PROVIDER: GSE91023 | GEO | 2017/01/16

SECONDARY ACCESSION(S): PRJNA356692

REPOSITORIES: GEO

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