Genomics

Dataset Information

0

Copy number variant (CNV) and Single nucleotide polymorphism (SNP) of UCLA hESC lines


ABSTRACT: Female human ESC-lines can carry active X-chromosomes (Xa) or an XIST-RNA- coated inactive X-chromosome (Xi XIST+ ). Additionally, many ESC-lines have abnormal X-chromosome-inactivation (XCI)-states where the Xi no longer expresses XIST-RNA and has transcriptionally active regions (eroded Xi=Xe). The fate of each XCI-state upon differentiation is unclear because individual lines often contain a mixture of XCI-states. Here, we established homogeneous XiXa, XeXa, and XaXa ESC-lines. We found that these lines were unable to initiate XIST-expression and X-chromosome- wide silencing upon differentiation indicating that the ESC XCI-state is maintained in differentiated cells. Consequently, differentiated XeXa and XaXa cells displayed higher levels of X-linked gene-expression than XiXa cells. Although global transcriptional compensation between X-chromosomes and autosomes is not required for female ESC-differentiation, the degree of X-chromosome- silencing influences differentiation efficiencies. Our data suggest that the Xi XIST+ Xa state is inherent to human ESCs and that all other XCI-states, including XaXa, are abnormal and arise during ESC-derivation or maintenance.

ORGANISM(S): Homo sapiens

PROVIDER: GSE91072 | GEO | 2016/12/15

SECONDARY ACCESSION(S): PRJNA356825

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2017-01-26 | GSE88932 | GEO
2017-01-26 | GSE88931 | GEO
2010-11-10 | E-GEOD-20937 | biostudies-arrayexpress
2010-05-28 | E-GEOD-21141 | biostudies-arrayexpress
2010-03-19 | GSE20937 | GEO
2010-05-28 | GSE21141 | GEO
2023-08-09 | GSE213979 | GEO
2021-09-30 | GSE163319 | GEO
2021-09-30 | GSE163320 | GEO
2013-11-04 | E-GEOD-48649 | biostudies-arrayexpress