Transcriptomics

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Transcriptome Profiling of bending fruit and Functional Analysis of an ERF/AP2 Family Gene CsERF025 Involved in bending fruit development in cucumber (Cucumis sativus L.)


ABSTRACT: To identify the differentially expressed genes(DEGs) in the ventral and dorsal of cucumber bending fruits,cucumber bending fruit mRNA profiles of 2-day-old ventral and dorsal were generated by deep sequencing. After removing the low quality reads, the total number of clean reads in two library were 27.07 million and16.52 million, accounted for 85.21% and 80.71% of total reads .We identified a total of 4313 sequences differentially expressed in ventral and dorsal of bending fruit with a 2-fold or greater change and P < 0.01 , the Dorsal (D2) served as a control, in which 2351 up-regulated genes and 1962 down-regulated genes.For up-regulated genes, protein kinase activity, ethylene mediated signaling pathway, regulation of cell shape, auxin polar transport were significantly enriched, whereas down-regulated genes, the functional classes photosynthesis, oxidation reduction, response to auxin stimulus were significantly enriched. Moreover, among DEGs related to ethylene, we identified an ERF/AP2 gene CsERF025 that was significant difference in the dorsal and ventral of cucumber. up-regulation of CsERF025 in cucumber promoted fruit bendng and Increased the fruit bending angle, suggesting that CsERF025 plays an important role in cucumber bending fruit.

ORGANISM(S): Cucumis sativus

PROVIDER: GSE91085 | GEO | 2019/12/07

REPOSITORIES: GEO

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