Transcriptomics

Dataset Information

0

Astrocyte-specific transcriptome responses to chronic ethanol consumption


ABSTRACT: Purpose: Traditional whole-tissue sequencing approaches do not fully capture brain cell-type specific effects of chronic alcohol. Therefore, the purpose of this study was to identify the specific transcriptome alterations in astrocytes due to chronic alcohol. Methods: We performed RNA-sequencing on astrocytes isolated from the prefrontal cortex (PFC) of C57BL/6J mice following chronic every-other-day alcohol consumption. Results: Differential expression analysis revealed alcohol-induced gene expression changes unique to astrocytes that could not be identified using whole tissue homogenate analysis. Enrichment analysis revealed involvement of calcium-related signaling and regulation of extracellular matrix genes in the astrocyte response to alcohol abuse. Conclusion: Our study presents the first focused analysis on the astrocyte transcriptome following chronic alcohol consumption, provides a framework for studying the functional response of astrocytes to alcohol and the possible astrocyte-specific effects of alcohol. In addition, our data represents a novel resource for groups interested in biological functions of astrocytes in the adult mouse PFC.

ORGANISM(S): Mus musculus

PROVIDER: GSE92457 | GEO | 2018/01/10

SECONDARY ACCESSION(S): PRJNA357612

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2020-06-01 | GSE132516 | GEO
2019-08-20 | GSE128561 | GEO
2023-05-15 | MTBLS5071 | MetaboLights
2020-11-27 | GSE142571 | GEO
2022-12-21 | GSE192522 | GEO
2013-10-28 | E-GEOD-51730 | biostudies-arrayexpress
| PRJNA357612 | ENA
| PRJNA356884 | ENA
2017-05-18 | MODEL1608180000 | BioModels
2020-11-24 | GSE161986 | GEO