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Seq-Well: A portable, low-cost platform for single-cell RNA-seq of low-input samples

ABSTRACT: Single-cell RNA -seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA -seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We characterize Seq-Well extensively and use it to profile thousands of primary human macrophages exposed to tuberculosis.

OTHER RELATED OMICS DATASETS IN: PRJNA374551

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE92495 | GEO | 2017/02/17

SECONDARY ACCESSION(S): PRJNA374551

REPOSITORIES: GEO

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Seq-Well: A portable, low-cost platform for single-cell RNA-seq of low-input samples

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