Dataset Information

Gene expression analysis of neuropathic pain-induced depression in the anterior cingulate cortex of mice

ABSTRACT: We used the sciatic nerve cuffing model to induce neuropathic pain and depression in mice. Gene expression was studied in the anterior cingulate cortex, a brain region crucial for mood disorders. We studied gene expression after 2 and 8 weeks in cuff or sham operated mice. The 8 week time point corresponded to the presence of both nociceptive and anxiodepressive-like phenotype. The 2 week time point correspond to the animals displaying no anxiodepressive phenotype.

ORGANISM(S): Mus musculus

PROVIDER: GSE92718 | GEO | 2019/12/11

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Neuropathic pain is a chronic debilitating condition with a high comorbidity with depression. Clinical reports and animal studies have suggested that both the medial prefrontal cortex (mPFC) and the anterior cingulate cortex (ACC) are critically implicated in regulating the affective symptoms of neuropathic pain. Neuropathic pain induces long-term structural, functional and biochemical changes in both regions, which are thought to be regulated by multiple waves of gene transcription. However, the similarity and differences in the transcriptomic profiles changed by neuropathic pain between these regions are largely unknown. Furthermore, women are more susceptible to pain and depression than men. The molecular mechanisms underlying this sexual dimorphism remain to be explored. Here, we performed RNA sequencing and analyzed the transcriptomic profiles of the mPFC and ACC of female and male mice at 2 weeks after spared nerve injury (SNI), an early time point when the mice began to show mild depressive symptoms. Our results show that the SNI-induced transcriptomic changes in female and male mice are largely distinct. Interestingly, the female mice exhibit more robust transcriptomic changes in the ACC than male, whereas the opposite pattern occurs in the mPFC. Cell type enrichment analyses reveal that the differentially expressed genes involve genes enriched in both neurons and various types of glia. We further performed Gene Set Enrichment Analysis (GSEA), which reveal significant de-enrichment of myelin sheath development in both female and male mPFC after SNI. In the female ACC, gene sets for synaptic organization and mitochondria function are enriched, and gene sets for extracellular matrix are de-enriched after SNI, while such signatures are absent in male ACC. Collectively, these findings reveal sexual dimorphism at the transcriptional level induced by neuropathic pain, and provide novel therapeutic targets for chronic pain and its associated affective disorders.

Dataset Information

Gene expression analysis of neuropathic pain-induced depression in the anterior cingulate cortex of mice

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets