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Gene expression profiling of human MSC-educated macrophages vs. classical macrophages from bone marrow and blood

ABSTRACT: In comparison to MØs, MEMs have increased expression of the inhibitory molecules PD-L1, PD-L2, in addition to markers of alternatively activated macrophages: CD206 and CD163. RNA-Seq analysis of MEMs, as compared to MØs, show a distinct gene expression profile that positively correlates with multiple pathways important in tissue repair. MEMs also show increased expression of IL-6, TGF-β, Arginase-1, CD73, and decreased expression of IL-12 and TNF-α. We show that IL-6 secretion is controlled in part by the COX-2, arginase and JAK1/STAT1 pathway. When tested in vivo, we show that human MEMs significantly enhance survival from lethal GVHD, and improve survival of mice from radiation injury.

ORGANISM(S): Homo sapiens

PROVIDER: GSE93155 | GEO | 2017/03/22

SECONDARY ACCESSION(S): PRJNA360146

REPOSITORIES: GEO

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