Transcriptomics

Dataset Information

0

RNA-Sequencing of Wild Type, Lola-F and Lola-Null mutant embryos


ABSTRACT: longitudinals lacking (lola) is among the most complex genes in Drosophila melanogaster, encoding up to 20 different protein isoforms and acting as a key transcription factor in axonal pathfinding and neural reprograming. To better characterize Lola function we have generated specific mutations in each isoform using the CRISPR/Cas9 system. Our targeted screen allows us to revisit the previously demonstrated roles for few isoforms, to assign known functions to specific isoforms and to reveal a critical role for a specific variant in the octopaminergic pathway. Thus, our comprehensive study expands the repertoire of Lola functions, and demonstrates that the CRISPR/Cas9 approach is a valuable tool to systematically address the role of complex loci in vivo.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE93294 | GEO | 2020/01/08

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2017-12-15 | GSE97836 | GEO
2020-09-11 | GSE103700 | GEO
2020-09-11 | GSE103709 | GEO
2020-09-11 | GSE103701 | GEO
2013-08-28 | E-GEOD-50372 | biostudies-arrayexpress
| PRJNA404083 | ENA
2013-08-11 | E-GEOD-49772 | biostudies-arrayexpress
2013-08-11 | E-GEOD-49773 | biostudies-arrayexpress
2019-09-07 | GSE136999 | GEO
2014-05-06 | E-GEOD-53447 | biostudies-arrayexpress