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Effect of BCL11B knockdown on transcriptome of human T-cell precursors

ABSTRACT: To investigate the role of BCL11B in the initial stages of human thymopoiesis, we performed loss of function (knockdown) studies in an in vitro human thymopoiesis model (cord blood CD34+ cells co-cultured on OP9DLL1 stromal cell line). Gene expression profiling by RNA-Seq demonstrated that BCL11B knockdown resulted in downregulation of T-lineage genes and upregulation of stem cell, myeloid and NK genes, indicating BCL11B is required for the establishment of a T-lineage commitment transcriptional program.

ORGANISM(S): Homo sapiens

PROVIDER: GSE93902 | GEO | 2017/03/06

SECONDARY ACCESSION(S): PRJNA362787

REPOSITORIES: GEO

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BCL11B DNA binding sites in progenitor and differentiated populations in the human thymus

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