Other

Dataset Information

0

Circularized RNA sequencing of wild-type and Mrpp3 knock out mouse heart mitochondria


ABSTRACT: Here we find that circularization of RNA prior to deep sequencing enables the discovery and characterization of unprocessed mitochondrial RNAs. Using this approach we identify the most stable processing intermediates and the presence of intermediate processing products that are partially degraded and polyadenylated. Analysis of libraries constructed using RNA from mice lacking the nuclease subunit of the mitochondrial RNase P reveals the identities of stalled processing intermediates, their order of cleavage, and confirms the importance of RNase P (MRPP3) in generating mature mitochondrial RNAs. Using RNA circularization prior to library preparation should provide a generally useful approach to studying RNA processing in many different biological systems.

ORGANISM(S): Mus musculus

PROVIDER: GSE94030 | GEO | 2017/03/23

SECONDARY ACCESSION(S): PRJNA363082

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2016-08-04 | E-GEOD-83471 | biostudies-arrayexpress
2024-11-11 | GSE267078 | GEO
2024-11-11 | GSE267077 | GEO
2016-08-04 | GSE83471 | GEO
2017-03-23 | GSE94005 | GEO
2008-01-01 | GSE6767 | GEO
2021-12-23 | GSE192549 | GEO
2016-06-28 | E-GEOD-75247 | biostudies-arrayexpress
2016-06-28 | E-GEOD-75410 | biostudies-arrayexpress
2016-06-28 | GSE75410 | GEO